Impact of flooding on soil bacterial communities associated with poplar (Populus sp.) trees
| Autor: | Ralf Conrad, Andrea Graff |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Bulk soil Applied Microbiology and Biotechnology Microbiology Plant Roots Disasters Salicaceae Gene Frequency RNA Ribosomal 16S Botany Phylogeny Soil Microbiology Rhizosphere Ecology biology Bacteria fungi Genes rRNA Sequence Analysis DNA biology.organism_classification Terminal restriction fragment length polymorphism Populus Microbial population biology Species richness Microcosm Soil microbiology Polymorphism Restriction Fragment Length |
| Zdroj: | FEMS microbiology ecology. 53(3) |
| ISSN: | 0168-6496 |
| Popis: | Soil bacterial communities were analyzed in different habitats (bulk soil, rhizosphere, rhizoplane) of poplar tree microcosms (Populus tremulaxP. alba) using cultivation-independent methods. The roots of poplar trees regularly experience flooded and anoxic conditions. Therefore, we also determined the effect of flooding on microbial communities in microcosm experiments. Total community DNA was extracted and bacterial 16S rRNA genes were amplified by PCR and analyzed by terminal restriction fragment length polymorphism (T-RFLP) analysis, cloning and sequencing. Clone libraries were created from all three habitats under both unflooded and flooded conditions resulting in a total of 281 sequences. Numbers of different sequences ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text