Casp3/7-Instructed Intracellular Aggregation of Fe3O4 Nanoparticles Enhances T2 MR Imaging of Tumor Apoptosis
| Autor: | Junchao Qian, Kai Zhong, Jia Zhang, Jinyong Xu, Tao Han, Shuchao Ge, Yuwei Wang, Yufeng Luo, Yue Yuan, Gaolin Liang, Xuejiao Dong, Zhanling Ding |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Nanoparticle Apoptosis Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Caspase 7 Imaging phantom Nuclear magnetic resonance In vivo Neoplasms medicine Humans General Materials Science Magnetite Nanoparticles health care economics and organizations medicine.diagnostic_test Caspase 3 Mechanical Engineering technology industry and agriculture Magnetic resonance imaging Hep G2 Cells General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Magnetic Resonance Imaging In vitro Neoplasm Proteins 0104 chemical sciences Biophysics Magnetic nanoparticles 0210 nano-technology Intracellular |
| Zdroj: | Nano Letters. 16:2686-2691 |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | Large magnetic nanoparticles or aggregates are advantageous in their magnetic resonance properties over ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles (NPs), but the former are cleared faster from the blood pool. Therefore, the "smart" strategy of intracellular aggregation of USPIO NPs is required for enhanced T2-weighted MR imaging. Herein, employing an enzyme-instructed condensation reaction, we rationally designed a small molecule Ac-Asp-Glu-Val-Asp-Cys(StBu)-Lys-CBT (1) to covalently modify USPIO NPs to prepare monodispersive Fe3O4@1 NPs. In vitro results showed that Fe3O4@1 NPs could be subjected to caspase 3 (Casp3)-instructed aggregation. T2 phantom MR imaging showed that the transverse molar relaxivity (r2) of Fe3O4@1 NPs with Casp3 or apoptotic HepG2 cells was significantly larger than those of control groups. In vivo tumor MR imaging results indicated that Fe3O4@1 NPs could be specifically applied for enhanced T2 MR imaging of tumor apoptosis. We propose that the enzyme-instructed intracellular aggregation of Fe3O4 NPs could be a novel strategy for the design of "smart" probes for efficient T2 MR imaging of in vivo biomarkers. |
| Databáze: | OpenAIRE |
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