Human Mesenchymal Stromal Cell Enhanced Morphological Polarization by Contact Interaction with Polyethylene Terephthalate Nanogratings
| Autor: | Paolo Domenico Parchi, Sandro Meucci, Simone Pacini, Fabio Beltram, Orazio Vittorio, Michele Lisanti, Martino Alfredo Cappelluti, Sara Antonini, Emanuela Jacchetti, Marco Cecchini, Mario Petrini |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Mechano-trasduction
Stromal cell Materials science Nanogratings Human Mesenchymal Stromal Cell Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Nanotechnology chemistry.chemical_compound Polyethylene terephthalate Mechanotransduction Cell adhesion Cytoskeleton Bone regeneration mechanotransduction Polyethylene Terephthalate Mesenchymal stem cell chemistry micrograting Biophysics nanograting Surface modification mesenchymal stromal cells Biotechnology |
| Zdroj: | Current nanoscience info:cnr-pdr/source/autori:Antonini, Sara[ 1,2 ] ; Cappelluti, Martino Alfredo[ 1,2 ] ; Meucci, Sandro[ 1,2,3 ] ; Jacchetti, Emanuela[ 1,2 ] ; Vittorio, Orazio[ 1,2 ] ; Parchi, Paolo[ 4 ] ; Lisanti, Michele[ 4 ] ; Pacini, Simone[ 5 ] ; Petrini, Mario[ 5 ] ; Beltram, Fabio[ 1,2 ] ; Cecchini, Marco[ 1,2 ]/titolo:Human Mesenchymal Stromal Cell Enhanced Morphological Polarization by Contact Interaction with Polyethylene Terephthalate Nanogratings/doi:/rivista:Current nanoscience (Print)/anno:2014/pagina_da:773/pagina_a:778/intervallo_pagine:773–778/volume:10 ResearcherID Scopus-Elsevier |
| Popis: | Understanding how the substrate topography acts on human bone marrow-derived mesenchymal stromal cells (MSCs) can help the rational design of scaffolds for improving bone regeneration protocols. MSCs are highly sensitive to the extracellular physical properties and can be successfully manipulated by simple contact interaction with supporting substrates. To this end, some polymeric materials were introduced, but polyethylene terephthalate (PET), a thermoplastic polymer approved by the US Food and Drug Administration for clinical use and very attractive in terms of biocompatibil- ity and mechanical properties, has not been tested yet in terms of cell mechanotransduction. Here, we propose PET nanogratings (alternating lines of submicron ridges and grooves) as scaffolds for stimulating mechanotransduction mechanisms. Low-temperature hot embossing is exploited as fabrication method, and standard oxygen plasma activation as functionalization to improve cell adhesion and spreading. We show that the substrate directionality stimulus is opti- mally delivered to the MSCs, which in turn elongate and align to the nanograting lines. Finally, we verify that this polari- zation occurs also at level of cytoskeleton fibers and, though to a lesser extent, of nuclei. |
| Databáze: | OpenAIRE |
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