Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights
| Autor: | Haikel Jelassi, Ahmed Salaheddine Masmoudi, Ameur Cherif, Habib Chouchane, Mohamed Neifar, Haïtham Sghaier, Sihem Guesmi, Wafa Hassen, Sahar Boutiti, Awatef Ouertani |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
Polymers and Plastics medicine.medical_treatment Organic chemistry genomic analyses Article gamma-irradiated derivatives chemistry.chemical_compound QD241-441 native exopolysaccharide Biosynthesis In vivo antioxidant activities medicine Gene chemistry.chemical_classification Halomonas desertis G11 Halomonas biology Chemistry fungi General Chemistry biology.organism_classification In vitro Enzyme Biochemistry Cell culture antitumor activities |
| Zdroj: | Polymers Volume 13 Issue 21 Polymers, Vol 13, Iss 3798, p 3798 (2021) |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym13213798 |
| Popis: | In this work, a native exopolysaccharide (nEPS) produced by Halomonas desertis G11 isolated from a Tunisian extreme environment was modified by gamma irradiation. Characterization as well as the antioxidant and antitumor activities of nEPS and its gamma-irradiated derivatives (iEPSs) were comparatively evaluated. In vitro and in vivo antioxidant potentials were determined by using different methods and through different antioxidant enzymes. The antitumor activity was checked against a human colon cancer cell line. Analyses of the complete genome sequence were carried out to identify genes implicated in the production of nEPS. Thus, the genomic biosynthesis pathway and the export mechanism of nEPS were proposed. Analyses of irradiation data showed that iEPSs acquired new functional groups, lower molecular weights, and gained significantly (p < 0.05) higher antioxidant and antitumor abilities compared with nEPS. These findings provide a basis for using iEPSs as novel pharmaceutical agents for human therapies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |