Relationship between anti-Müllerian hormone and antral follicle count across the menstrual cycle using the Beckman Coulter Access assay in comparison with Gen II manual assay
| Autor: | Christian Gnoth, Julia Schiffner, Judith Roos, Daniel Fehr, Günter Freundl, David Broomhead, Erhard Godehardt, Sarah Johnson, Joseph van Helden |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Anti-Mullerian Hormone 0301 basic medicine medicine.medical_specialty Adolescent media_common.quotation_subject Clinical Biochemistry Biology Luteal phase Young Adult 03 medical and health sciences 0302 clinical medicine Ovarian Follicle Internal medicine Follicular phase medicine Humans Ovarian follicle Ovulation Menstrual Cycle Menstrual cycle media_common 030219 obstetrics & reproductive medicine Biochemistry (medical) Anti-Müllerian hormone General Medicine Antral follicle 030104 developmental biology Endocrinology medicine.anatomical_structure biology.protein Female Blood Chemical Analysis Hormone |
| Zdroj: | Clinical Chemistry and Laboratory Medicine (CCLM). 55 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm-2016-0609 |
| Popis: | Background:The study aim was to validate Beckman Coulter’s fully automated Access Immunoassay System (BC Access assay) for anti-Müllerian hormone (AMH) and compare it with Beckman Coulter’s Modified Manual Generation II assay (BC Mod Gen II), with regard to cycle AMH fluctuations and antral follicle counts.Methods:During one complete menstrual cycle, transvaginal ultrasound was performed on regularly menstruating women (n=39; 18–40years) every 2 days until the dominant ovarian follicle reached 16mm, then daily until observed ovulation; blood samples were collected throughout the cycle. Number and size of antral follicles was determined and AMH levels measured using both assays.Results:AMH levels measured by the BC Access assay vary over ovulatory menstrual cycles, with a statistically significant pre-ovulatory decrease from –5 to +2 days around objective ovulation. Mean luteal AMH levels were significantly lower (–7.99%) than mean follicular levels but increased again towards the end of the luteal phase. Antral follicle count can be estimated from AMH (ng/mL, BC Access assay) concentrations on any follicular phase day. BC Access assay-obtained AMH values are considerably lower compared with the BC Mod Gen II assay (–19% on average); conversion equation: AMH BC Access (ng/mL)=0.85 [AMH BC Mod Gen II (ng/mL)]Conclusions:AMH levels vary throughout the cycle, independently of assay utilised. A formula can be used to convert BC Access assay-obtained AMH levels to BC Mod Gen II values. The number of antral follicles can be consistently estimated from pre-ovulatory AMH levels using either assay. |
| Databáze: | OpenAIRE |
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