Cross-reactive Neutralizing Antibody Responses to Enterovirus 71 Infections in Young Children: Implications for Vaccine Development
| Autor: | Mei Liang Huang, Luan-Yin Chang, Min Shi Lee, Tzou Yien Lin, Shu Ting Luo, Pai Shan Chiang, Min Yuan Chia, Mei-Shang Ho |
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| Rok vydání: | 2013 |
| Předmět: |
Serotype
lcsh:Arctic medicine. Tropical medicine Genotype lcsh:RC955-962 viruses Molecular Sequence Data Taiwan Cross Reactions Biology Antibodies Viral medicine.disease_cause Pediatrics Serology Viral Proteins Antigen Enterovirus Infections Enterovirus 71 medicine Antigenic variation Humans Neutralizing antibody Sequence Homology Amino Acid lcsh:Public aspects of medicine Public Health Environmental and Occupational Health virus diseases lcsh:RA1-1270 Viral Vaccines Sequence Analysis DNA biology.organism_classification Antibodies Neutralizing Virology Enterovirus A Human Infectious Diseases Child Preschool biology.protein Medicine RNA Viral Enterovirus Antibody Research Article |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Vol 7, Iss 2, p e2067 (2013) |
| ISSN: | 1935-2735 |
| Popis: | Background Recently, enterovirus 71 (EV71) has caused life-threatening outbreaks involving neurological and cardiopulmonary complications in Asian children with unknown mechanism. EV71 has one single serotype but can be phylogenetically classified into 3 main genogroups (A, B and C) and 11 genotypes (A, B1∼B5 and C1∼C5). In Taiwan, nationwide EV71 epidemics with different predominant genotypes occurred in 1998 (C2), 2000–2001 (B4), 2004–2005 (C4), and 2008 (B5). In this study, sera were collected to measure cross-reactive neutralizing antibody titers against different genotypes. Methods We collected historical sera from children who developed an EV71 infection in 1998, 2000, 2005, 2008, or 2010 and measured cross-reactive neutralizing antibody titers against all 11 EV71 genotypes. In addition, we aligned and compared the amino acid sequences of P1 proteins of the tested viruses. Results Serology data showed that children infected with genogroups B and C consistently have lower neutralizing antibody titers against genogroup A (>4-fold difference). The sequence comparisons revealed that five amino acid signatures (N143D in VP2; K18R, H116Y, D167E, and S275A in VP1) are specific for genogroup A and may be related to the observed antigenic variations. Conclusions This study documented antigenic variations among different EV71 genogroups and identified potential immunodominant amino acid positions. Enterovirus surveillance and vaccine development should monitor these positions. Author Summary Recently, enterovirus 71 (EV71) has caused life-threatening outbreaks in tropical Asia. EV71 has one single serotype but can be phylogenetically classified into 3 main genogroups and 11 genotypes (A, B1∼B5 and C1∼C5). In Taiwan, nationwide EV71 epidemics with different predominant genotypes occurred in 1998(C2), 2000–2001(B4), 2004–2005(C4), and 2008(B5). In this study, historical sera from children infected with these 4 genotypes were collected to measure cross-reactive neutralizing antibody titers against 11 genotypes. In addition, amino acid sequences of P1 proteins of the tested viruses were compared. Serology data showed that children infected with genogroup B and C consistently have lower neutralizing antibody titers against genogroup A (>4-fold difference). Antigenic variations between genogroup B and C could be detected but did not have a clear pattern. Five amino acid signatures are specific for genogroup A and may be related to the observed antigenic variations. Vaccine development should monitor the antigenic and genetic variations to select vaccine strains. |
| Databáze: | OpenAIRE |
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