Quercetin inhibits lymphocyte activation and proliferation without inducing apoptosis in peripheral mononuclear cells
| Autor: | Lara Gibellini, Leonarda Troiano, Roberta Ferraresi, Elisa Nemes, Milena Nasi, Paolo Salomoni, Edwin L. Cooper, Erika Roat, Linda Bertoncelli, Marcello Pinti, Enrico Lugli, Andrea Cossarizza |
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| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
T cell Quercetin Leukemic cell lines PBMCs Apoptosis Lymphocyte function Biology Lymphocyte Activation Peripheral blood mononuclear cell Immunophenotyping Flow cytometry Immune system Downregulation and upregulation medicine Humans heterocyclic compounds Cell Proliferation U937 cell medicine.diagnostic_test U937 Cells Hematology Flow Cytometry Cell biology medicine.anatomical_structure Oncology Cell culture Cancer research |
| Zdroj: | Leukemia Research. 33:140-150 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/j.leukres.2008.07.025 |
| Popis: | Toxicity of chemotherapeutic drugs towards normal cells is a serious side effect of cancer treatment. Thus, finding of molecules with low toxicity for normal cells is crucial. Several natural compounds, such as flavonoid quercertin, are receiving a growing attention as "chemopreventers". Quercetin kills tumour-derived cell lines, but little is known about its effects on normal cells. Here we show that although quercetin exerts a higher apoptotic potential on leukemic cell lines than on peripheral blood mononuclear cells (PBMCs) and does not sensitize PBMCs to CD95-induced apoptosis, it is able to inhibit normal immune functions such as T cell proliferation and activation. Quercetin sensitivity is independent on cell cycle progression since it was not abrogated in serum-starved U937 cells, nor proliferating PBMCs underwent apoptosis after quercetin treatment. However, quercetin prevented PHA-induced PBMC proliferation and SEB-induced upregulation of activation markers. Our data suggest that quercetin, while incapable of inducing apoptosis in normal cells under several conditions, could interfere with effector T cell function. |
| Databáze: | OpenAIRE |
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