Design and Development of Biomimetic Nanovesicles Using a Microfluidic Approach
| Autor: | Stefano Alcaro, Donatella Paolino, Francesca Taraballi, Michael B. Sherman, Kelly A. Hartman, Michael Evangelopoulos, Jessica R. Hoffman, Isabella Romeo, Jonathan O. Martinez, Giosuè Costa, Claudia Corbo, Ennio Tasciotti, Roberto Molinaro, Donato Cosco |
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| Přispěvatelé: | Molinaro, R, Evangelopoulos, M, Hoffman, J, Corbo, C, Taraballi, F, Martinez, J, Hartman, K, Cosco, D, Costa, G, Romeo, I, Sherman, M, Paolino, D, Alcaro, S, Tasciotti, E |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Microfluidics microfluidic Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Drug Delivery Systems Biomimetics Biological property General Materials Science bioinspired approach molecular dynamic Mechanical Engineering 021001 nanoscience & nanotechnology molecular dynamics 0104 chemical sciences membrane protein incorporation Mechanics of Materials inflammation Drug delivery microfluidics Nanoparticles 0210 nano-technology microfluidics bio-inspired nanoparticles |
| Zdroj: | Advanced materials (Deerfield Beach, Fla.). 30(15) |
| ISSN: | 1521-4095 |
| Popis: | The advancement of nanotechnology toward more sophisticated bioinspired approaches has highlighted the gap between the advantages of biomimetic and biohybrid platforms and the availability of manufacturing processes to scale up their production. Though the advantages of transferring biological features from cells to synthetic nanoparticles for drug delivery purposes have recently been reported, a standardizable, batch-to-batch consistent, scalable, and high-throughput assembly method is required to further develop these platforms. Microfluidics has offered a robust tool for the controlled synthesis of nanoparticles in a versatile and reproducible approach. In this study, the incorporation of membrane proteins within the bilayer of biomimetic nanovesicles (leukosomes) using a microfluidic-based platform is demonstrated. The physical, pharmaceutical, and biological properties of microfluidic-formulated leukosomes (called NA-Leuko) are characterized. NA-Leuko show extended shelf life and retention of the biological functions of donor cells (i.e., macrophage avoidance and targeting of inflamed vasculature). The NA approach represents a universal, versatile, robust, and scalable tool, which is extensively used for the assembly of lipid nanoparticles and adapted here for the manufacturing of biomimetic nanovesicles. |
| Databáze: | OpenAIRE |
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