Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize
| Autor: | Hai-lin Xiao, Yan-xin Zhao, Jiang-hua Yu, Bing Yue, Ya-ting Qin, Yonglian Zheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Solexa sequencing
CMS-S Agriculture (General) Endogeny Plant Science Biology medicine.disease_cause maize Biochemistry S1-972 Food Animals Pollen microRNA Transcriptional regulation medicine Gene Genetics Ecology target gene Cytoplasmic male sterility microRNAs Real-time polymerase chain reaction Cytoplasm Animal Science and Zoology real-time PCR Agronomy and Crop Science Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 12, Iss 2, Pp 229-238 (2013) |
| ISSN: | 2095-3119 |
| Popis: | MicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value1.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes. |
| Databáze: | OpenAIRE |
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