The slow resorption with replacement by bone of a hydrothermally synthesized pure calcium-deficient hydroxyapatite
Autor: | Koji Ioku, Hideyuki Minagi, Yasuaki Shibata, Yoshinori Gonda, Tohru Ikeda, Masanobu Kamitakahara, Ikuho Yonezawa, Takatoshi Okuda, Hisashi Kurosawa, Hisashi Murayama, Giichiro Kawachi |
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Rok vydání: | 2008 |
Předmět: |
Materials science
Biophysics Osteoclasts chemistry.chemical_element Biocompatible Materials Bioengineering Calcium Bone tissue Cell Line Biomaterials Mice Osteogenesis In vivo Osteoclast medicine Animals Humans Femur Bone Resorption Tomography X-Ray Anatomy Molecular biology Resorption Durapatite medicine.anatomical_structure chemistry Mechanics of Materials Cell culture Bone Substitutes Microscopy Electron Scanning Ceramics and Composites Alkaline phosphatase Female Rabbits Bone marrow |
Zdroj: | Biomaterials. 29:2719-2728 |
ISSN: | 0142-9612 |
Popis: | A newly developed calcium-deficient hydroxyapatite composed of rod-shaped particles synthesized by the hydrothermal method (HHA) and stoichiometric hydroxyapatite (SHA) synthesized by the sintering method was used for in vivo implantation and in vitro culture systems to compare these biological responses. In the rabbit femur, implanted HHA was slowly resorbed and about 80% of the implant remained 24 weeks after implantation; however, up to 72 weeks after implantation, most of the implanted HHA was resorbed. The implanted SHA was unresorbed throughout the experimental period, but degradation by the invasion of newly formed bone was seen at 72 weeks after implantation. Bone histomorphometry showed that the volume of newly formed bone and the number of osteoclasts in the implanted region were significantly higher in HHA than in SHA 24 weeks after implantation. In vitro culture of C2C12 cells with the induction of osteoblastic phenotypes using recombinant bone morphogenetic protein-2 showed similar cell density and the induction of alkaline phosphatase activity between the cells on HHA and SHA discs. In vitro osteoclastogenesis of HHA and SHA discs using bone marrow macrophages and recombinant receptor activator of nuclear factor-κB ligand showed higher TRAP activity of osteoclasts cultured on HHA discs. These results showed that slow biodegradability did not always correlate to final replaceability in bone tissue, and suggested that the activity of osteoclasts correlated to the bone-forming activity of osteoblasts. |
Databáze: | OpenAIRE |
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