Amphotericin B-loaded polymeric nanoparticles: formulation optimization by factorial design
Autor: | Rubiana Mara Mainardes, Talita Cristina Moreira Moraes Carraro, Najeh Maissar Khalil |
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Rok vydání: | 2014 |
Předmět: |
Polymers
Chemistry Pharmaceutical Dispersity Ethyl acetate Pharmaceutical Science Nanoparticle macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Polyethylene Glycols chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Amphotericin B Organic chemistry Lactic Acid Particle Size chemistry.chemical_classification Drug Carriers technology industry and agriculture General Medicine Polymer Factorial experiment 021001 nanoscience & nanotechnology 0104 chemical sciences PLGA chemistry Chemical engineering Nanoparticles Particle size Methanol 0210 nano-technology Polyglycolic Acid |
Zdroj: | Pharmaceutical development and technology. 21(2) |
ISSN: | 1097-9867 |
Popis: | In this study, PLGA or PLGA-PEG blend nanoparticles were developed loading amphotericin B (AmB), an antifungal agent broadly used in therapy. A 2(2) × 3(1) factorial experimental design was conducted to indicate an optimal formulation of nanoparticles containing AmB and demonstrate the influence of the interactions of components on the mean particle size and drug encapsulation efficiency. The independent variables analyzed were polymer amount (two levels) and organic phase (three factors in one level). The parameters methanol as cosolvent and higher polymer amount originated from the higher AmB encapsulation, but with the larger particle size. The selected optimized parameters were set as the lower polymer amount and ethyl acetate as cosolvent in organic phase, for both PLGA and PLGA-PEG nanoparticles. These parameters originated from nanoparticles with the size of 189.5 ± 90 nm and 169 ± 6.9 nm and AmB encapsulation efficiency of 94.0 ± 1.3% and 92.8 ± 2.9% for PLGA and PLGA-PEG nanoparticles, respectively. Additionally, these formulations showed a narrow size distribution indicating homogeneity in the particle size. PLGA and PLGA-PEG nanoparticles are potential carrier for AmB delivery and the factorial design presented an important tool in optimizing nanoparticles formulations. |
Databáze: | OpenAIRE |
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