Quantitative analysis of C60 fullerene in blood and tissues by high-performance liquid chromatography with photodiode-array and mass spectrometric detection
| Autor: | Fathi Moussa, Eric Genin, François Trivin, André Rassat, Stéphane Roux, René Céolin, H. Szwarc, Monique Pressac |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Mass spectrometry High-performance liquid chromatography Antiviral Agents Sensitivity and Specificity law.invention chemistry.chemical_compound Mice law Spectrophotometry medicine Animals Chromatography High Pressure Liquid Detection limit Chromatography medicine.diagnostic_test Reproducibility of Results General Chemistry Mass spectrometric Carbon Drug Residues Photodiode Circadian Rhythm Specific Pathogen-Free Organisms chemistry Liver C70 fullerene Linear Models Spectrophotometry Ultraviolet Fullerenes Quantitative analysis (chemistry) Injections Intraperitoneal Spleen |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 696(1) |
| ISSN: | 1387-2273 |
| Popis: | A high-performance liquid chromatographic (HPLC) assay method for C60 fullerene, in blood, liver and spleen using photodiode-array detection or mass spectrometric detection (LC-MS) and C70 fullerene, as the internal standard, is described. The recovery from mouse blood and tissues spiked with micronized C60 exceeds 90%. The method is linear from 0.05 to 200 mg of C60 per liter of blood and from 0.05 to 5.00% of C60 per tissue weight. The limit of detection of the method is 0.1 ng of C60 per injection. This method was applied to mouse blood and tissue samples after intraperitoneal administration of a micronized C60 suspension. |
| Databáze: | OpenAIRE |
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