Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
| Autor: | Agnaldo Fernando Baldo da Silva, Osvaldo de Freitas, Jonas Augusto Rizzato Paschoal, Fabiana Pilarski, Pedro Garcia F. Neto, Evandro Bilha Moro, Mark Mooney |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Queen's University Belfast |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Drug
food.ingredient Food Safety 030309 nutrition & dietetics media_common.quotation_subject Aquaculture Veterinary drug residues Commercial fish feed Food safety 03 medical and health sciences 0404 agricultural biotechnology food Oral administration Tandem Mass Spectrometry Animals Food science Emamectin benzoate media_common 0303 health sciences Ivermectin TILÁPIA biology business.industry Chemistry Muscles Fish fillet Tilapia 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Animal Feed Drug Residues Residual depletion Oreochromis Withdrawal period business Food Science Chromatography Liquid |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Made available in DSpace on 2019-10-06T16:15:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-05-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Due to the lack of regulated drugs for aquaculture, the present study considered specific issues relating to environmental and food safety aspects concerning the potential use of emamectin benzoate (EMA) in freshwater fishes such as tilapia (Oreochromis niloticus) – an important commercial fish species worldwide. The residual depletion of EMA (EMA-B 1a ) in fillet (muscle plus skin in natural proportions) of tilapia treated with a daily dose of 50 μg/kg BW during seven consecutive days was evaluated. To facilitate this, analytical methods for quantitation of EMA in fish feed and in fish fillet employing LC-MS/MS were developed and validated. To eliminate the risk of EMA leaching from feed into the aquatic environment during fish medication via oral administration, a promising procedure for drug incorporation into feed involving the coating of feed pellets with ethyl cellulose polymer containing EMA was evaluated. The medicated feed exhibited good homogeneity (CV < 2.1%) with negligible EMA release (< LOQ) when the medicated feed remained in the water for up to 20 min. Depletion study analysis revealed the highest EMA concentration obtained in fish fillet to be 13.3 ng/g. Therefore, under the employed rearing conditions of this study, the obtained results did not evidence requirement for a minimum withdrawal period to be proposed considering the maximum residue limit of 100 μg/g for fish muscle. In response to the well-recognized demands and need for new alternative veterinary drugs for use within aquaculture, this study offers impetus for consideration of EMA use in tilapia taking into account environmental contamination and food safety issues. Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Física e Química Universidade de São Paulo (USP) Centro de Aquacultura da Unesp Universidade do Estado de São Paulo (Unesp) Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Ciências Farmacêuticas Universidade de São Paulo (USP) Institute for Global Food Security School of Biological Sciences Queen's University Belfast Centro de Aquacultura da Unesp Universidade do Estado de São Paulo (Unesp) FAPESP: 2012/18334-0 FAPESP: 2014/50755-0 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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