The Newly Identified Yeast GRD Genes Are Required for Retention of Late-Golgi Membrane Proteins
| Autor: | S F Nothwehr, Tom H. Stevens, Nia J. Bryant |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
musculoskeletal diseases
Recombinant Fusion Proteins Genes Fungal Molecular Sequence Data Saccharomyces cerevisiae Golgi Apparatus Vacuole Peptidyl-Dipeptidase A Biology digestive system Fungal Proteins symbols.namesake stomatognathic system Amino Acid Sequence Molecular Biology Fungal protein musculoskeletal neural and ocular physiology Genetic Complementation Test Membrane Proteins Cell Biology Golgi apparatus Alkaline Phosphatase musculoskeletal system biology.organism_classification Yeast Transmembrane protein Complementation Phenotype Membrane protein Biochemistry Mutation Vacuoles symbols Protein Processing Post-Translational Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.16.6.2700 |
| Popis: | Processing of A-ALP, a late-Golgi membrane protein constructed by fusing the cytosolic domain of dipeptidyl aminopeptidase A to the transmembrane and lumenal domains of alkaline phosphatase (ALP), serves as a convenient assay for loss of retention of late-Golgi membrane proteins in Saccharomyces cerevisiae. In this study, a large group of novel grd (for Golgi retention defective) yeast mutants, representing 18 complementation groups, were identified on the basis of their mislocalization of A-ALP to the vacuole, where it was proteolytically processed and thus became enzymatically activated. All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its |
| Databáze: | OpenAIRE |
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