Expression of enhancer binding factors associated with various cell types of lung cancer
| Autor: | Yuk-Chor Wong, Samuel D. Bernal |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cell type
Lung Neoplasms Proto-Oncogene Proteins c-jun Molecular Sequence Data Biophysics Adenocarcinoma Biology Biochemistry Cell Line Enhancer binding medicine Carcinoma Humans Lung cancer Enhancer Molecular Biology Binding Sites Base Sequence Nuclear Proteins Cell Biology medicine.disease Squamous carcinoma DNA-Binding Proteins Enhancer Elements Genetic Oligodeoxyribonucleotides Cell culture Immunology Cancer research Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 180:1064-1069 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(05)81174-x |
| Popis: | Differential expression of nuclear factors binding specifically to AP-1, AP-2, AP-3, and NF-I/CTF enhancer elements was found in the various cell types of human lung carcinoma cells. Adenocarcinoma and squamous carcinoma cells seemed to express higher levels of these factors than large-cell carcinoma and small-cell carcinoma cells. Among small-cell carcinoma cells, variant small-cell carcinoma cells which presumably are closer than classical small-cell carcinoma cells to non-small-cell carcinoma cells in terms of differentiation characteristics also expressed higher levels of these factors. Furthermore, nuclear extracts from the various cell types of carcinoma cells were found to produce different patterns of electrophoretic mobility shift even with the same enhancer element, suggesting the presence of multiple species of specific enhancer-binding activities in these cells. Thus, these enhancer elements may be used as probes for cell-type specificity in the study of differentiation of lung carcinomas. |
| Databáze: | OpenAIRE |
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