Comparison of proteoglycans from bovine articular cartilage
| Autor: | Lawrence Rosenberg, William Strider, Subhash Pal |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Cartilage
Articular Macromolecular Substances Articular cartilage Ulna Biochemistry Genetics and Molecular Biology (miscellaneous) Guanidines medicine Animals Magnesium Aggrecan Glycosaminoglycans biology Chemistry Cartilage Anatomy Humerus carbohydrates (lipids) Molecular Weight medicine.anatomical_structure Proteoglycan Occipital Bone biology.protein Biophysics Cattle Proteoglycans Collagen Protein Binding |
| Zdroj: | Biochimica et biophysica acta. 379(1) |
| ISSN: | 0006-3002 |
| Popis: | Four bovine articular cartilages have been compared with regard to the chemical composition of the whole cartilages, the amount of proteoglycan selectively extracted with 3 M MgCl2 or with 3 M guanidine·HCl, and the compositions and physical properties of the isolated proteoglycans. The whole cartilages differ but slightly in composition. Occipital condylar cartilage, a thin cartilage from the smallest joint, contains 4% more collagen and proportionately less proteoglycan than proximal humeral, the thickest cartilage from the largest joint. Each cartilage contains a pool of proteoglycan that resists extraction with 3 M MgCl2 but is extracted with 3 M guanidine·HCl. The proteoglycan extracted from each cartilage with 3 M guanidine·HCl contains a high molecular weight proteoglycan-collagen complex demonstrated by analytical ultracentrifugation and by the turbidity of its visible and ultraviolet spectra. The four cartilages appear to differ most remarkably in the fraction of total proteoglycan extracted from each as proteoglycan-collagen complex. |
| Databáze: | OpenAIRE |
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