Pyridine Ruthenium(III) complexes entrapped in liposomes with enhanced cytotoxic properties in PC-3 prostate cancer cells
| Autor: | Giancarlo Morelli, Francesco Ruffo, Maria Elena Cucciolito, Roberta Iannitti, Rosanna Palumbo, Angela D'Amora, Diego Tesauro |
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| Přispěvatelé: | D'Amora, Angela, Cucciolito, Maria Elena, Iannitti, Roberta, Morelli, Giancarlo, Palumbo, Rosanna, Ruffo, Francesco, Tesauro, Diego |
| Rok vydání: | 2019 |
| Předmět: |
Liposome
Egg l-α-phosphatidylcholine cholesterol Chemistry PC-3 prostate cancer cell Pharmaceutical Science chemistry.chemical_element 02 engineering and technology 021001 nanoscience & nanotechnology Ligand (biochemistry) 030226 pharmacology & pharmacy In vitro Ruthenium 03 medical and health sciences DSPE-PEG liposome Inorganic complexes delivery 0302 clinical medicine Membrane Dynamic light scattering In vivo Pyridine ruthenium (III) complexe Biophysics Cytotoxic T cell 0210 nano-technology |
| Zdroj: | Journal of Drug Delivery Science and Technology. 51:552-558 |
| ISSN: | 1773-2247 |
| DOI: | 10.1016/j.jddst.2019.02.009 |
| Popis: | The first aim of the present study is the development of a new ruthenium(III) complex, belonging to NAMI-A class, with a potentially high cytotoxic ability. The presence of a fully protected sugar moiety as ruthenium ligand should increase the complex ability to cross cellular membranes. Furthermore, it sets this molecule in the area of biocompatible agents as tumor drug. The second, more relevant, purpose is to verify the ruthenium complexes activity after loading into liposomes. We reported the characterization and in vitro biological assays of pyridine derivatives of ruthenium complexes loaded into Egg l -α-phosphatidylcholine cholesterol/DSPE-PEG liposomes. Dynamic light scattering estimates that the sizes of all obtained liposomes are in the 100 nm range. This value is suitable for in vivo use. The loading ability and release kinetic allowed selecting the best ratio between the lipid fraction and metal to be tested in cellular experiments. The growth inhibitory effects of both liposomal and free complex in PC-3 prostate cancer cell lines demonstrate a high cytotoxic ability of the liposome entrapped ruthenium (III) complex suggesting additional role further the antimetastatic function. |
| Databáze: | OpenAIRE |
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