Quantitative determination of CGP 61755, a protease inhibitor, in plasma and urine by high-performance liquid chromatography and fluorescence detection
| Autor: | P.H. Degen, Ch Mann, Gerard Flesch |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Analyte
Coefficient of variation Administration Oral Urine Fluorescamine High-performance liquid chromatography Sensitivity and Specificity Fluorescence spectroscopy chemistry.chemical_compound Dogs Drug Stability Freezing Animals Humans Chromatography High Pressure Liquid Fluorescent Dyes Detection limit Chromatography Osmolar Concentration Reproducibility of Results General Chemistry HIV Protease Inhibitors Ethylenes Reference Standards Circadian Rhythm Rats Spectrometry Fluorescence chemistry Quantitative analysis (chemistry) |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 696(1) |
| ISSN: | 1387-2273 |
| Popis: | A liquid chromatographic assay for the determination of CGP 61755 (I) in plasma and urine is described. A similar method for CGP 53437, another HIV-1 protease inhibitor, has been developed and reported previously. After a deproteinization step, a liquid-liquid extraction is performed. Compound I and the internal standard CGP 55749 (II) are hydrolyzed and the primary amine group derivatized using fluorescamine. Chromatography is achieved by isocratic elution with a mobile phase of 30 mM borax buffer (pH 9)-acetonitrile (58:42, v/v). The derivatives of the compounds I and II fluoresce at 480 nm, on excitation at 395 nm and the retention times under these conditions were approximately 6 and 8 min, respectively. The limit of quantitation (LOQ) which is the lowest concentration of the analyte that can be measured with a coefficient of variation and a deviation from theory of less than 20%, was 15 ng/ml plasma and 20 ng/ml urine. The analyte is stable for at least four months in human plasma and sixteen months in dog plasma samples. Different human plasma sources and three different species (rat, rabbit and dog) were tested and no interference between analyte and plasma constituents was observed. |
| Databáze: | OpenAIRE |
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