Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
| Autor: | Rubens Spin-Neto, Andreas Stavropoulos, Guilherme José Pimentel Lopes de Oliveira, Cássio Rocha Scardueli, Fernanda Castanheira Gonçalves, Rosemary Adriana Chierici Marcantonio |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Uberlândia (UFU), Section of Oral Radiology, Malmo University |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Time Factors Bone Regeneration medicine.medical_treatment Bone Morphogenetic Protein 2 Odontologi Bone Regeneration/drug effects Skull/drug effects Random Allocation 0302 clinical medicine Osteogenesis General Materials Science 030212 general & internal medicine Saline Bone substitutes biology Biomaterial RK1-715 Immunohistochemistry Osteocalcin/analysis Osteogenesis/drug effects Bone regeneration medicine.anatomical_structure Osteocalcin Cyclosporine musculoskeletal diseases medicine.medical_specialty Calcineurin Inhibitors Calvaria Bone healing Bone morphogenetic protein 2 Calcineurin Inhibitors/pharmacology Transforming Growth Factor beta1 03 medical and health sciences Internal medicine medicine Animals Bone Substitutes/pharmacology business.industry Skull Reproducibility of Results 030206 dentistry X-Ray Microtomography Rats Transforming Growth Factor beta1/analysis Endocrinology Cyclosporine/pharmacology Dentistry Bone Morphogenetic Protein 2/analysis Bone Substitutes biology.protein business Immunosuppression |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Oral Research, Vol 34 (2020) Gonçalves, F C, Oliveira, G J P L D, Scardueli, C R, Spin-Neto, R, Stavropoulos, A & Marcantonio, R A C 2020, ' Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes ', Brazilian Oral Research, vol. 34, e007 . https://doi.org/10.1590/1807-3107bor-2020.vol34.0007 Brazilian Oral Research, Volume: 34, Article number: e007, Published: 07 FEB 2020 Brazilian Oral Research v.34 2020 Brazilian Oral Research Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
| Popis: | Made available in DSpace on 2020-12-12T01:56:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 The aim of this study was to assess the influence of cyclosporine administration on the repair of critical-sized calvaria defects (CSDs) in rat calvaria filled with diverse biomaterials. Sixty animals were divided into two groups: the control (CTR) group (saline solution) and the cyclosporine (CCP) group (cyclosporine, 10 mg/kg/day). These medications were administered daily by gavage, beginning 15 days before the surgical procedure and lasting until the day the animals were euthanized. A CSD (5 mm O) was made in the calvaria of each animal, which was allocated to one of 3 subgroups, according to the biomaterial used to fill the defect: coagulum (COA), deproteinized bovine bone (DBB), or biphasic calcium phosphate ceramics of hydroxyapatite and β-phosphate tricalcium (HA/TCP). Euthanasia of the animals was performed 15 and 60 days after the surgical procedure (n = 5 animals/period/subgroup). Bone repair (formation) assessment was performed through microtomography and histometry, while the analyses of the expression of the BMP2, Osteocalcin, and TGFβ1 proteins were performed using immunohistochemistry. The CSDs not filled with biomaterials demonstrated lower bone formation in the CCP group. At 15 days, less bone formation was observed in the CSDs filled with DBB, a smaller volume of mineralized tissue was observed in the CSDs filled with HA/TCP, and the expression levels of BMP2 and osteocalcin were lower in the CCP group compared to the CTR group. The use of cyclosporine impaired bone repair in CSD, and this effect can be partially explained by the suppression of BMP2 and osteocalcin expression. Universidade Estadual de São Paulo-Unesp School of Dentistry Araraquara Department of Diagnosis and Surgery Universidade Federal de Uberlândia-UFU Dental School Department of Periodontology Aarhus University Department of Dentistry and Oral Health Section of Oral Radiology Malmo University Department of Community Dentistry and Periodontology Universidade Estadual de São Paulo-Unesp School of Dentistry Araraquara Department of Diagnosis and Surgery |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|