Synthesis, Structural and Micromechanical Properties of 3D Hyaluronic Acid-Based Cryogel Scaffolds
| Autor: | Claude Oelschlaeger, Norbert Willenbacher, Frank Bossler |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microrheology Materials science Compressive Strength Polymers and Plastics Biocompatible Materials Bioengineering 02 engineering and technology Viscoelasticity Polymerization Biomaterials 03 medical and health sciences Rheology Elastic Modulus Materials Testing Polymer chemistry Materials Chemistry medicine Hyaluronic Acid Particle Size Elastic modulus chemistry.chemical_classification Tissue Scaffolds Viscosity Swelling capacity Polymer Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Kinetics 030104 developmental biology chemistry Chemical engineering Particle size Swelling medicine.symptom 0210 nano-technology Porosity Cryogels |
| Zdroj: | Biomacromolecules. 17:580-589 |
| ISSN: | 1526-4602 1525-7797 |
| Popis: | In this study, macroporous, elastic, three-dimensional scaffolds formed of hyaluronic acid mixed with ethylene glycol diglycidyl ether as a chemical cross-linker have been prepared by cryogelation for application in tissue engineering. These cryogels are characterized by large interconnected pores of size ∼50-300 μm and pore wall thickness of ∼5-30 μm as determined from confocal microscopy images. Variation of pH, freezing temperature, and polymerization time allows for control of pore size and shape as well as matrix thickness. These structural properties then determine mechanical strength as well as swelling capacity. Furthermore, increasing hyaluronic acid concentration decreases cryogel pore size, reduces swelling properties, and reinforces mechanical properties. On the other hand, decreasing cross-linker concentration, at a constant hyaluronic acid concentration, increases pore size and swelling capacity but provides less rigidity. Additionally, for the first time, local elastic properties of the polymer matrix and viscous properties of the pores have been characterized using multiple particle tracking microrheology. Local matrix elasticity, relaxation time of hyaluronic acid chains, and the degree of heterogeneity are discussed in detail. These latter properties are crucial for the development of new tissue engineering constructs and will help to understand how local matrix viscoelasticity affects cell cultivation. Finally, elastic moduli obtained in bulk rheology are much higher than corresponding values deduced from microrheology. This discrepancy might be explained by the formation of very highly cross-linked cores in the network where no tracer particle can penetrate. |
| Databáze: | OpenAIRE |
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