Leaf shape in Populus tremula is a complex, omnigenic trait
| Autor: | Nathaniel R. Street, Mark E.S. Bailey, Stefan Jansson, Chanaka Mannapperuma, Torgeir R. Hvidsten, Barbara K Terebieniec, Kathryn M. Robinson, Matej Vucak, Bastian Schiffthaler, Niklas Mähler |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Candidate gene Population Context (language use) Genome-wide association study Bioinformatik och systembiologi Biology leaf shape 010603 evolutionary biology 01 natural sciences 03 medical and health sciences lcsh:QH540-549.5 GWAS natural variation education Populus tremula Gene Ecology Evolution Behavior and Systematics Original Research 030304 developmental biology Nature and Landscape Conservation 0303 health sciences education.field_of_study Bioinformatics and Systems Biology Ecology fungi food and beverages Genetic architecture complex trait Evolutionary biology Expression quantitative trait loci Trait lcsh:Ecology omnigenic |
| Zdroj: | Ecology and Evolution Ecology and Evolution, Vol 10, Iss 21, Pp 11922-11940 (2020) |
| ISSN: | 2045-7758 |
| DOI: | 10.1002/ece3.6691 |
| Popis: | Leaf shape is a defining feature of how we recognize and classify plant species. Although there is extensive variation in leaf shape within many species, few studies have disentangled the underlying genetic architecture. We characterized the genetic architecture of leaf shape variation in Eurasian aspen (Populus tremula L.) by performing genome‐wide association study (GWAS) for physiognomy traits. To ascertain the roles of identified GWAS candidate genes within the leaf development transcriptional program, we generated RNA‐Seq data that we used to perform gene co‐expression network analyses from a developmental series, which is publicly available within the PlantGenIE resource. We additionally used existing gene expression measurements across the population to analyze GWAS candidate genes in the context of a population‐wide co‐expression network and to identify genes that were differentially expressed between groups of individuals with contrasting leaf shapes. These data were integrated with expression GWAS (eQTL) results to define a set of candidate genes associated with leaf shape variation. Our results identified no clear adaptive link to leaf shape variation and indicate that leaf shape traits are genetically complex, likely determined by numerous small‐effect variations in gene expression. Genes associated with shape variation were peripheral within the population‐wide co‐expression network, were not highly connected within the leaf development co‐expression network, and exhibited signatures of relaxed selection. As such, our results are consistent with the omnigenic model. We characterized the genetic architecture of leaf shape variation in Eurasian aspen (Populus tremula L.) by performing a genome‐wide association studies (GWAS) for physiognomy traits. Our results identified no clear adaptive link to leaf shape variation and indicate that leaf shape traits are genetically complex, likely determined by numerous small‐effect variations in gene expression. Genes associated with shape variation were peripheral within the population‐wide co‐expression network, were not highly connected within the leaf development co‐expression network, and exhibited signatures of relaxed selection. |
| Databáze: | OpenAIRE |
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