ER-MP12 antigen, a new cell surface marker on mouse bone marrow cells with thymus-repopulating ability: II. Thymus-homing ability and phenotypic characterization of ER-MP12-positive bone marrow cells
| Autor: | Dale I. Godfrey, W. Van Ewijk, J. C. M. Van Der Loo, Walentina A. T. Slieker, M. F. T. R. De Bruijn, Pieter J. M. Leenen |
|---|---|
| Přispěvatelé: | Immunology, Hematology |
| Rok vydání: | 1993 |
| Předmět: |
Myeloid
T-Lymphocytes Immunology Bone Marrow Cells Thymus Gland Biology Immunotherapy Adoptive Immunophenotyping Mice Antigen Bone Marrow medicine Animals Immunology and Allergy Progenitor cell B cell Mice Inbred BALB C Antibodies Monoclonal General Medicine Flow Cytometry Hematopoietic Stem Cells Molecular biology Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Antigens Surface Mice Inbred CBA Bone marrow Biomarkers Homing (hematopoietic) |
| Zdroj: | International Immunology, 5(9), 1099-1107. Oxford University Press |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/5.9.1099 |
| Popis: | In the accompanying paper we showed that six distinct subsets of bone marrow (BM) cells can be identified using the mAb ER-MP12 and ER-MP20 in two-colour immunofluorescence analysis. Upon intrathymic transfer into sublethally irradiated mice thymus-repopulating ability was restricted to ER-MP20- BM cells expressing either high or intermediate levels of the ER-MP12 antigen (1-2% and -30% of BM nucleated cells respectively). The highest frequency of thymus-repopulating cells was found in the minor subset of ER-MP12++20- BM cells. In the present study we demonstrate that upon intravenous transfer, thymus-homing and-repopulating BM cells are exclusively confined to the ER-MP12++20- and ER-MP12+20- subpopulations, the highest frequency being detected among ER-MP12++20- BM cells. Analysis of the peripheral blood leucocytes of reconstituted mice showed that not only prothymocytes but also progenitorcells of the B cell lineage as well as the myelold lineage were present within both subsets. Three-colour flow cytometric analysis revealed that ER-MP12++20- BM cells in particular were phenotyplcally heterogeneous with respect to the expression of the cell surface markers Thy-1, Sca-1, CD44, B220 and c-kit. Taken together our data demonstrate that ER-MP12 positively identifies BM cells with the ability to home to and repopulate the thymus. The phenotypic heterogeneity displayed by the ER-MP12++20- BM subset, containing the highest frequency of thymus-homing and-repopulating cells, provides a basis for further separation of prothymocyte activity from other haematopoietic activities in the BM of the mouse. |
| Databáze: | OpenAIRE |
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