Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil

Autor: Dik Mevius, Marita Vedovelli Cardozo, Lucas José Luduverio Pizauro, Apostolos Liakopoulos, Michael S.M. Brouwer, Mariana Monezi Borzi, Fernando Antonio de Ávila, Arie Kant
Přispěvatelé: Universidade Estadual Paulista (UNESP), Wageningen University and Research
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Microbiology (medical)
plasmids
animal structures
antibiotic resistance
Klebsiella pneumoniae
Epidemiology
Bioinformatica & Diermodellen
Biology
medicine.disease_cause
Microbiology
03 medical and health sciences
Plasmid
Bio-informatics & Animal models
medicine
Epidemiology
Bio-informatics & Animal models
Typing
Escherichia coli
030304 developmental biology
Southern blot
Original Research
Phylotype
Human feces
Host Pathogen Interaction & Diagnostics
Epidemiologie
0303 health sciences
030306 microbiology
poultry
Bacteriologie
extended spectrum beta lactamases (ESBLs)
Bacteriology
Bacteriology
Host Pathogen Interaction & Diagnostics
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
bacterial infections and mycoses
Host Pathogen Interactie & Diagnostiek
QR1-502
food chain
Epidemiologie
Bioinformatica & Diermodellen
Bacteriologie
Host Pathogen Interactie & Diagnostiek
Multilocus sequence typing
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Frontiers in Microbiology
Frontiers in Microbiology, 12
Frontiers in Microbiology, Vol 12 (2021)
Frontiers in Microbiology 12 (2021)
ISSN: 1664-302X
Popis: Made available in DSpace on 2022-04-28T19:44:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-22 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the blaCTX–M–1 or blaCTX–M–2 gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The blaCTX–M genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of blaCTX–M–15 among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America. Department of Veterinary Pathology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) Department of Bacteriology and Epidemiology Wageningen Bioveterinary Research Wageningen University and Research Department of Tecnology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) Department of Veterinary Pathology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) Department of Tecnology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) FAPESP: 2013/18280-0 FAPESP: 2015/10140-0
Databáze: OpenAIRE
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