Moderate diabetes alters myosin isoenzyme distribution in cardiac but not skeletal muscle of male rats
| Autor: | G.S Morris, Arnold G. Nelson, Prevost Mc |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Blood Glucose
Male Gene isoform Vacuolar Proton-Translocating ATPases medicine.medical_specialty Glycosylation ATPase Myosins General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Rats Sprague-Dawley Hemoglobins Isomerism Diabetes mellitus Internal medicine Myosin medicine Animals General Pharmacology Toxicology and Pharmaceutics Muscle Skeletal Adenosine Triphosphatases Myosin Heavy Chains biology Myocardium Skeletal muscle General Medicine medicine.disease Streptozotocin Rats Isoenzymes Phenotype Endocrinology medicine.anatomical_structure biology.protein MYH7 MYH6 medicine.drug |
| Zdroj: | Life Sciences. 58:833-838 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(96)00016-1 |
| Popis: | Diabetes is known to alter the myosin phenotype of striated muscle, but the impact of the same diabetic state on different types of striated muscles remains unknown. Therefore, this study determined the myosin isoenzyme profile in the left ventricle, soleus, plantaris, and extensor digitorium longus (EDL) of young male rats made moderately diabetic with streptozotocin, (45 mg/kg, ip). Eight weeks after the single streptozotocin injection, tissues were collected and subsequently electrophoretically analyzed for native myosin isoenzyme distribution. Skeletal muscles were additionally analyzed for myosin heavy chain distribution. Neither the native myosin isoform nor the myosin heavy chain (MHC) distribution profiles of the skeletal muscles were altered by the diabetic state. In contrast, the high ATPase cardiac isoform, VI, was significantly replaced by the low ATPase isoform, V3 (p0.05). These results demonstrate that striated muscle responds to a moderate diabetic state in a limited and muscle specific fashion. Significantly, the change in the cardiac myosin isoform profile is comparable to that which occurs in a more severe diabetic state. |
| Databáze: | OpenAIRE |
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