Neurotransmitters, KCl and antioxidants rescue striatal neurons from apoptotic cell death in culture
| Autor: | Natalie D. Stull, Amanda Mishizen, Lorraine Iacovitti |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Cell Survival medicine.medical_treatment Apoptosis Antioxidants Culture Media Serum-Free Membrane Potentials Potassium Chloride Mice chemistry.chemical_compound Internal medicine Dopamine receptor D2 Glial cell line-derived neurotrophic factor medicine Animals Neurotransmitter Molecular Biology Cells Cultured Neurons Neurotransmitter Agents biology General Neuroscience Growth factor Depolarization Corpus Striatum Monoamine neurotransmitter Endocrinology nervous system chemistry Dopamine Agonists biology.protein Neurology (clinical) Developmental Biology |
| Zdroj: | Brain Research. 816:276-285 |
| ISSN: | 0006-8993 |
| Popis: | Striatal neurons grown in low density culture on serum-free media and in the absence of glia die within 3 days of plating. In this study, we sought to determine the mechanism of cell death (e.g., apoptosis) and whether trophic influences, such as, growth factors, neurotransmitters, antioxidants or KCl-mediated depolarization could improve their survival. We found that striatal neurons grown in this manner die via apoptosis unless treated with one of several different rescuing agents. One way to prevent the death of most striatal neurons was continual treatment with 5-20 microM dopamine (DA) or other monoamines. Although the survival effect of DA was mimicked by the specific D1 receptor agonist, SKF38393, no D1 or D2 receptor antagonists blocked the effect. As with DA, chronic depolarization with KCl (12-39 mM) or treatment with antioxidants, such as the vitamin E analog, Trolox (10-10-500 microM), or the hormone, melatonin (10-10-500 microM) also rescued striatal neurons from impending cell death. Surprisingly, growth factors, such as BDNF, bFGF, GDNF, NGF, NT3 and EGF, demonstrated no ability to rescue striatal neurons in this model, suggesting that death was not solely caused by the absence of essential trophic factors. We conclude that a variety of agents, but not growth factors, can prevent the demise of striatal neurons, presumably by neutralizing damage at one or more steps in the death cascade. |
| Databáze: | OpenAIRE |
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