Hyperosmotic and isosmotic shrinkage differentially affect protein phosphorylation and ion transport
| Autor: | Ryszard Grygorczyk, Sergei V. Kotelevtsev, Svetlana V. Koltsova, Sergei N. Orlov, Olga A. Akimova |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Physiology Myocytes Smooth Muscle Biology Muscle Smooth Vascular Dogs Osmotic Pressure Physiology (medical) Internal medicine medicine Animals Mitogen-Activated Protein Kinase 9 Mitogen-Activated Protein Kinase 8 Protein phosphorylation Intercalated Cell Kidney Tubules Collecting Phosphorylation Na+/K+-ATPase Aorta Ion transporter Cell Size Shrinkage Mitogen-Activated Protein Kinase 1 Pharmacology Ion Transport Mitogen-Activated Protein Kinase 3 Osmotic concentration Epithelial Cells Sodium-Phosphate Cotransporter Proteins General Medicine Rats Kinetics Endocrinology Biophysics Sodium-Potassium-Exchanging ATPase Cotransporter Protein Processing Post-Translational |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 90:209-217 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/y11-119 |
| Popis: | In the present work, we compared the outcome of hyperosmotic and isosmotic shrinkage on ion transport and protein phosphorylation in C11-MDCK cells resembling intercalated cells from collecting ducts and in vascular smooth muscle cells (VSMC) from the rat aorta. Hyperosmotic shrinkage was triggered by cell exposure to hypertonic medium, whereas isosmotic shrinkage was evoked by cell transfer from an hypoosmotic to an isosmotic environment. Despite a similar cell volume decrease of 40%–50%, the consequences of hyperosmotic and isosmotic shrinkage on cellular functions were sharply different. In C11-MDCK and VSMC, hyperosmotic shrinkage completely inhibited Na+,K+-ATPase and Na+,Picotransport. In contrast, in both types of cells isosmotic shrinkage slightly increased rather than suppressed Na+,K+-ATPase and did not change Na+,Picotransport. In C11-MDCK cells, phosphorylation of JNK1/2 and Erk1/2 mitogen-activated protein kinases was augmented in hyperosmotically shrunken cells by ∼7- and 2-fold, respectively, but was not affected in cells subjected to isosmotic shrinkage. These results demonstrate that the data obtained in cells subjected to hyperosmotic shrinkage cannot be considered as sufficient proof implicating cell volume perturbations in the regulation of cellular functions under isosmotic conditions. |
| Databáze: | OpenAIRE |
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