The RNA-binding protein SND1 promotes the degradation of GPX4 by destabilizing the HSPA5 mRNA and suppressing HSPA5 expression, promoting ferroptosis in osteoarthritis chondrocytes
| Autor: | Min Lv, Yuanzhen Cai, Weikun Hou, Kan Peng, Ke Xu, Chao Lu, Wenxing Yu, Weisong Zhang, Lin Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
Tumor Necrosis Factor-alpha Immunology Interleukin-1beta RNA-Binding Proteins Endonucleases Phospholipid Hydroperoxide Glutathione Peroxidase Rats MicroRNAs Chondrocytes Matrix Metalloproteinase 13 Osteoarthritis Animals Ferroptosis RNA Messenger Reactive Oxygen Species Cells Cultured Heat-Shock Proteins |
| Zdroj: | Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 71(4) |
| ISSN: | 1420-908X |
| Popis: | Heat shock protein family A member 5 (HSPA5), a recently identified suppressor of ferroptosis, was reported to potentially regulating osteoarthritis. However, the exact role of HSPA5 and how its expression was regulated in osteoarthritis are largely unclear.Rat primary chondrocytes were treated with 10 ng/mL IL-1β for 24 h and incubated with ferrostatin-1 (a ferroptosis inhibitor). Cell viability, production of TNF-α, ROS and MDA, expression levels of collagen II, MMP13, GPX4, and SND1, and FeIL-1β treatment could enhance extracellular matrix (ECM) degradation (collagen II reduced and MMP13 increased), promote ferroptosis, manifested by decreased cell viability, increased levels of TNF-α, ROS, MDA, and FeThe RNA-binding protein SND1 promotes the degradation of GPX4 by destabilizing the HSPA5 mRNA and suppressing HSPA5 expression, promoting ferroptosis in osteoarthritis chondrocytes. |
| Databáze: | OpenAIRE |
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