Differential Viral-Host Immune Interactions Associated with Oseltamivir-Resistant H275Y and Wild-Type H1N1 A(pdm09) Influenza Virus Pathogenicity
| Autor: | Inmaculada Casas, Natàlia Majó, Lorena Córdoba, Mónica Pérez, Jaime Martorell, Jorge Martínez, Pamela Martínez-Orellana, Beatriz Vidaña, Lorenzo Fraile, Lourdes Migura-Garcia, Massimiliano Baratelli, Maria Montoya, Francisco Pozo |
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| Přispěvatelé: | Instituto de Salud Carlos III, CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Universidad Autónoma de Barcelona, Generalitat de Catalunya, Producció Animal, Sanitat Animal, Instituto de Salud Carlos III - ISCIII |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine viruses lcsh:QR1-502 Virus Replication medicine.disease_cause lcsh:Microbiology Madin Darby Canine Kidney Cells Mice chemistry.chemical_compound Influenza A Virus H1N1 Subtype Immunopathology Influenza A virus immunopathology Lung Mutation Virulence virus diseases Infectious Diseases Female influenza Oseltamivir oseltamivir 030106 microbiology Mutation Missense Biology Antiviral Agents Article Virus resistance 03 medical and health sciences Dogs Immune system Orthomyxoviridae Infections Virology Drug Resistance Viral medicine Animals pneumonia Gene Host Microbial Interactions Ferrets Wild type pH1N1 Pneumonia Mice Inbred C57BL 030104 developmental biology chemistry Genetic Fitness |
| Zdroj: | IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Vidana Mateo, B V, Martínez-Orellana, P, Martorell, J, Baratelli, M, Martínez, J, García Migura, L, Cordoba, L, Pérez, M, Casas, I, Pozo, F, Fraile, L, Majó, N & Montoya, M 2020, ' Differential Viral-Host Immune Interactions Associated with Oseltamivir-Resistant H275Y and Wild-Type H1N1 A(pdm09) Influenza Virus Pathogenicity ', Viruses, vol. 12, no. 8, 794 . https://doi.org/10.3390/v12080794 Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Repositorio Abierto de la UdL Universitad de Lleida Repisalud Instituto de Salud Carlos III (ISCIII) Viruses Volume 12 Issue 8 Viruses, Vol 12, Iss 794, p 794 (2020) Recercat. Dipósit de la Recerca de Catalunya instname Digital.CSIC. Repositorio Institucional del CSIC Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| DOI: | 10.3390/v12080794 |
| Popis: | Oseltamivir is a common therapy against influenza A virus (IAV) infections. The acquisition of oseltamivir resistance (OR) mutations, such as H275Y, hampers viral fitness. However, OR H1N1 viruses have demonstrated the ability to spread throughout different populations. The objective of this work was to compare the fitness of two strains of OR (R6 and R7) containing the H275Y mutation, and a wild-type (F) pandemic influenza A (H1N1) 2009 (pdm09) virus both in vitro and in vivo in mice and to select one OR strain for a comparison with F in ferrets. R6 showed faster replication and pathogenicity than R7 in vitro and in mice. Subsequently, R6 was selected for the fitness comparison with the F strain in ferrets. Ferrets infected with the F virus showed more severe clinical signs, histopathological lung lesions, and viral quantification when compared to OR R6-infected animals. More importantly, differential viral kinetics correlated with differential pro-inflammatory host immune responses in the lungs of infected ferrets, where OR-infected animals developed a protective higher expression of type I IFN and Retinoid acid Inducible Gene I (RIG-I) genes early after infection, resulting in the development of milder disease. These results suggest the presence of early specific viral-host immune interactions relevant in the development of influenza-associated lung pathology. This work was funded by the coordinated project RTA 2011-00111-C03 of the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), by Instituto de Salud Carlos III (“Programa especial de investigación sobre la gripe pandémica” GR09/0023, GR09/0040, GR09/0039), and by the Servei de Diagnòstic de Patologia Veterinària (SDPV) of the Universitat Autònoma de Barcelona. This work was also partially funded by the CERCA program from Generalitat de Catalunya. |
| Databáze: | OpenAIRE |
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