Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay
| Autor: | László Galgóczy, Mónica Gandía, Jose F. Marcos, Anant Kakar, Florentine Marx, Pedro Martínez-Culebras, Moisés Giner-Llorca, Paloma Manzanares, Jeanett Holzknecht |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Generalitat Valenciana, European Commission, National Research, Development and Innovation Office (Hungary), Austrian Science Fund |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Penicillium italicum QH301-705.5 Plant Science Penicillium digitatum Article 03 medical and health sciences postharvest protection medicine Food science Biology (General) Penicillium expansum Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Penicillium decay biology 030306 microbiology Chemistry Blue mold food and beverages biology.organism_classification Penicillium chrysogenum Postharvest protection Fungicide medicine.drug_formulation_ingredient PAFB antifungal protein Penicillium Postharvest |
| Zdroj: | Journal of Fungi, Vol 7, Iss 449, p 449 (2021) Digital.CSIC. Repositorio Institucional del CSIC instname Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Journal of Fungi Volume 7 Issue 6 |
| Popis: | Penicillium phytopathogenic species provoke severe postharvest disease and economic losses. Penicillium expansum is the main pome fruit phytopathogen while Penicillium digitatum and Penicillium italicum cause citrus green and blue mold, respectively. Control strategies rely on the use of synthetic fungicides, but the appearance of resistant strains and safety concerns have led to the search for new antifungals. Here, the potential application of different antifungal proteins (AFPs) including the three Penicillium chrysogenum proteins (PAF, PAFB and PAFC), as well as the Neosartorya fischeri NFAP2 protein to control Penicillium decay, has been evaluated. PAFB was the most potent AFP against P. digitatum, P. italicum and P. expansum, PAFC and NFAP2 showed moderate antifungal activity, whereas PAF was the least active protein. In fruit protection assays, PAFB provoked a reduction of the incidence of infections caused by P. digitatum and P. italicum in oranges and by P. expansum in apples. A combination of AFPs did not result in an increase in the efficacy of disease control. In conclusion, this study expands the antifungal inhibition spectrum of the AFPs evaluated, and demonstrates that AFPs act in a species-specific manner. PAFB is a promising alternative compound to control Penicillium postharvest fruit decay. This research was funded by grant RTI2018-101115B-C21 from the Spanish Ministerio de Ciencia, Innovación y Universidades (co-financed with FEDER funds) and by PROMETEO/2018/066 from ‘Conselleria d’Educació’ (Generalitat Valenciana, Comunitat Valenciana, Spain) to P.M.-C., J.F.M. and P.M., and by the Austrian Science Fund FWF (grant I3132-B21 and HOROS Doctoral Program, W-1253 DK HOROS) to F.M., L.G. was financed by the FK 134343 project of the Hungarian National Research, Development and Innovation (NKFIH) Office. Research of L.G. has been supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. The present work of L.G. was supported by the ÚNKP-20-5—New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund. |
| Databáze: | OpenAIRE |
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