All-Trans Retinoic Acid Regulates Cx43 Expression, Gap Junction Communication and Differentiation in Primary Lens Epithelial Cells

Autor:	Deborah M. Grzybowski, Heather L. Chandler, Joshua A. Bomser, Amy C. Long
Rok vydání:	2010
Předmět:	Time Factors Cellular differentiation Immunoblotting Retinoic acid Connexin Tretinoin Cell Communication Biology Cellular and Molecular Neuroscience chemistry.chemical_compound Dogs Crystallin Lens Crystalline Animals RNA Messenger neoplasms Cells Cultured Cell Proliferation Messenger RNA Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction organic chemicals Gap junction Gap Junctions Cell Differentiation Epithelial Cells beta-Crystallins Molecular biology biological factors Sensory Systems Reverse transcription polymerase chain reaction Ophthalmology Gene Expression Regulation chemistry Connexin 43 Carbenoxolone sense organs Intracellular
Zdroj:	Current Eye Research. 35:670-679
ISSN:	1460-2202 0271-3683
DOI:	10.3109/02713681003770746
Popis:	To examine the effect of all-trans retinoic acid (ATRA) treatment on connexin 43 (Cx43) expression, gap junction intercellular communication (GJIC), and cellular differentiation in primary canine lens epithelial cells (LEC).Dose and time-dependent effects of ATRA on Cx43 protein, mRNA and GJIC, were assessed by immunoblotting, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and scrape loading/dye transfer assays, respectively. Expression of beta crystallin was evaluated by immunoblotting.Treatment with ATRA at non-cytotoxic concentrations significantly increased Cx43 protein, mRNA and GJIC in primary canine LEC. Treatment with ATRA for five and seven days increased levels of beta crystallin, a protein marker of LEC differentiation. Inhibition of GJIC via pre-treatment with a synthetic inhibitor, 18-alpha glycyrrethinic acid (AGA), reduced ATRA-induced increases in Cx43 and GJIC and partially blocked ATRA-induced beta crystallin protein.Treatment with ATRA significantly increased Cx43 expression and GJIC in canine LEC, and these effects were associated with increased LEC differentiation. Results from this study suggest that functional gap junctions may play a role in the modulation of cellular differentiation in primary canine LEC.
Databáze:	OpenAIRE
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