Evolutionary dynamics of endogenous Jaagsiekte sheep retroviruses proliferation in the domestic sheep, mouflon and Pyrenean chamois
| Autor: | Begoña M. Jugo, M Sistiaga-Poveda |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Lineage (genetic)
Virus Integration Molecular Sequence Data Population Endogenous retrovirus Genome Viral Virus Replication Evolution Molecular Phylogenetics Gene Order Genetics Animals education Phylogeny Sheep Domestic Genetics (clinical) education.field_of_study Polymorphism Genetic Sheep biology Inverse polymerase chain reaction Endogenous Retroviruses Terminal Repeat Sequences Computational Biology Genetic Variation Jaagsiekte sheep retrovirus Rupicapra biology.organism_classification Long terminal repeat Mouflon Original Article |
| Zdroj: | Heredity. 112:571-578 |
| ISSN: | 1365-2540 0018-067X |
| Popis: | The oncogenic exogenous Jaagsiekte sheep retrovirus (JSRV), responsible for ovine pulmonary adenocarcinoma, has several endogenous counterparts termed enJSRVs. Although many of these elements have been inactivated over time by the accumulation of deleterious mutations or internal recombination leading to solo long terminal repeat (LTR) formation, several members of enJSRVs have been identified as nearly intact and probably represent recent integration events. To determine the level of enJSRV polymorphism in the sheep population and related species, we have undertaken a study by characterizing enJSRVs copies and independent integration sites in six domestic sheep and two wild species of the sheep lineage. enJSRVs copies were detected by amplifying the env-LTR region by PCR, and for the detection of the insertion sites, we used two approaches: (1) an in silico approach based on the recently published Sheep Reference Genome Assembly (OARv3.0) and (2) an experimental approach based on PCR suppression and inverse PCR techniques. In total, 103 enJSRV sequences were generated across 10 individuals and enJSRV integrations were found on 11 of the 28 sheep chromosomes. These findings suggest that there are still uncharacterized enJSRVs, and that some of the integration sites are variable among the different species, breeds of the same species, subspecies and geographic locations. |
| Databáze: | OpenAIRE |
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