Grape seed proanthocyanidins induce apoptosis through the mitochondrial pathway in nasopharyngeal carcinoma CNE-2 cells
| Autor: | Caihong Li, Haitao Qiu, De Dai, Jingjing Shao, Fengxiang Cao, Kai Yao, Keyuan Zhou |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Cell cycle checkpoint Cell bcl-X Protein Apoptosis Biology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor medicine Humans Proanthocyanidins Viability assay bcl-2-Associated X Protein Membrane Potential Mitochondrial Nasopharyngeal Carcinoma Grape Seed Extract Caspase 3 Carcinoma Nasopharyngeal Neoplasms General Medicine Cell cycle medicine.disease Mitochondria G2 Phase Cell Cycle Checkpoints stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Oncology Nasopharyngeal carcinoma Cell culture 030220 oncology & carcinogenesis Immunology Cancer research Poly(ADP-ribose) Polymerases Signal Transduction |
| Zdroj: | Oncology Reports. 36:771-778 |
| ISSN: | 1791-2431 1021-335X |
| Popis: | Although modern radiotherapy offers excellent local control in the treatment of nasopharyngeal carcinoma (NPC), current therapeutic decisions remain burdensome due to the frequency of local recurrence and treatment failure at distant sites. One potential and promising strategy for the prevention or treatment of cancers is the use of bioactive components of plant origin, including dietary plant products. Herein, we studied one class of these bioactive compounds, grape seed proanthocyanidins (GSPs), and explored their effect on NPC CNE-2 cells, as well as the primary mechanism underlying this effect. Our results revealed that treatment of human NPC CNE-2 cells with GSPs reduced cell viability in a dose- and time-dependent manner, and moreover, markedly induced cell cycle arrest at the G2/M phase, leading to induction of apoptosis. In addition, we found that the underlying mechanism was associated with increased expression of the pro-apoptotic protein Bax, decreased expression of the anti-apoptotic proteins Bcl-2 and Bcl-xL, upregulation of cleaved caspase-3 and cleaved poly(ADP-ribose) polymerase (PRAP) protein, and the loss of mitochondrial membrane potential (MMP) (Δψm). Furthermore, GSPs upregulated the Bcl-2 homology 3 (BH3)-only proteins, Bim and Bad, in a concentration-dependent manner. Taken together, these data supported our hypothesis that, in human NPC CNE-2 cells, GSPs could induce apoptosis through the mitochondrial pathway and ultimately reduce cell viability. Collectively, the results discussed above provide substantive evidence for the potential of GSPs as an effective bioactive phytochemical for the treatment of NPC. |
| Databáze: | OpenAIRE |
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