Cell death in human atherosclerotic plaques involves both oncosis and apoptosis
| Autor: | Johan Thyberg, Bengt Kallin, Jan Nilsson, Milita Crisby, Vasilios Kostulas, Boris Zhivotovsky, Sten Orrenius |
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| Rok vydání: | 1997 |
| Předmět: |
Carotid Artery Diseases
Male Pathology medicine.medical_specialty Programmed cell death Necrosis Arteriosclerosis Cell Apoptosis DNA Fragmentation Biology Muscle Smooth Vascular medicine Humans Nick translation Aged Aged 80 and over Electrophoresis Agar Gel Cell Death Macrophages Fibrous cap Uterus Arteries Molecular biology Chromatin medicine.anatomical_structure Carotid Arteries DNA fragmentation Female medicine.symptom Cardiology and Cardiovascular Medicine |
| Zdroj: | Atherosclerosis. 130(1-2) |
| ISSN: | 0021-9150 |
| Popis: | The aim of the present study was to analyze the frequency and mechanism of cell death in atherosclerotic plaques with a recent history ( 70% diameter reduction) undergoing carotid endarterectomy. In situ tailing nick translation of fragmented DNA, agarose gel electrophoresis of plaque DNA and electron microscopy were used to identify cell death by apoptosis (programmed cell death) and necrosis. The mean number of cell containing fragmented DNA in the plaques was 12.7 ± 3.5% (n=15). Focal accumulations of cells with DNA fragmentation occurred in the fibrous cap, at sites of rupture, close to lipid deposits and necrosis and was always accompanied by the presence of inflammatory cells. Electrophoretic separation of DNA isolated form part of plaques where the presence of DNA fragmentation had previously been demonstrated by in situ DNA nick translation resulted in multiple ladders of 180–200 base pairs characteristic of apoptosis. Electron microscopic analysis revealed presence of cells with morphological signs of degeneration in a frequency even higher than that found by in situ nick translation. Some of these cells had a characteristic apoptotic appearance with condensed chromatin and cytoplasm, but the large majority of the cells had an ultrastructure typical for cells undergoing cell death by necrosis with membrane disruption and swollen, disintegrating organelles. Thus, although apoptosis clearly takes place in atherosclerotic plaques necrosis appears to be a much more common mechanism for cell death. This may have negative consequences for plaque stability since cell death by necrosis induces a stronger inflammatory reaction than apoptotic cell death. |
| Databáze: | OpenAIRE |
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