Application of Modified Shell Vial Culture Procedure for Arbovirus Detection
| Autor: | Tadeusz J. Kochel, Edna R. Caceda |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
viruses
Cell Culture Techniques Encephalitis Virus St. Louis lcsh:Medicine Alphavirus Dengue virus Biology medicine.disease_cause Models Biological complex mixtures Arbovirus Chemistry Techniques Analytical Virus Cell Line Microbiology Dengue fever Encephalitis Virus Venezuelan Equine fluids and secretions Species Specificity Chlorocebus aethiops medicine Animals lcsh:Science Vero Cells Multidisciplinary lcsh:R Reproducibility of Results Virology/Diagnosis Dengue Virus medicine.disease biology.organism_classification Virology digestive system diseases Titer Shell Vial Culture Saint Louis encephalitis lcsh:Q Yellow fever virus West Nile virus Arboviruses Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 2, Iss 10, p e1034 (2007) |
| ISSN: | 1932-6203 |
| Popis: | The isolation of arboviruses from patient's low titer sera can be difficult. Here we compared the detection efficiency of Dengue (DEN), Yellow Fever (YF), Saint Louis Encephalitis (SLE), West Nile (WN), Ilheus (ILH), Group C (GC), Oropouche (ORO), Mayaro (MAY) and Venezuela Encephalitis Equine (VEE) viruses using a Modified Shell Vial Culture (MSVC) protocol to a Standard Cell Culture (SCC) protocol. First the MSVC and SCC protocols were compared using five dilutions for each of the following stock viruses: DEN-1, DEN-2, DEN-3, DEN-4, YF, SLE, WN, ILH, GC, ORO, MAY and VEE. Next, patients' original sera from which viruses (DEN-1, DEN-2, DEN-3, YF, GC, ORO, MAY and VEE) had been previously isolated were compare by the two methods using five sera dilutions. In addition, seven sera that were positive for DEN-3 by RT-PCR and negative by SCC were processed by MSVC. The MSVC protocol was consistently 1-2 logs higher virus dilution more sensitive for virus detection than the SCC protocol for all stock Flaviviruses tested (DEN-1, DEN-2, DEN-3, DEN-4, YF, SLE, WN and ILH). MSVC was equal to or one log more sensitive for virus detection than SCC for the stock Bunyaviruses (GC and ORO). For the stock Alphavirus MAY, MSVC was equally or one log more sensitive for virus detection than SCC, while for VEE SCC was equally or one log more sensitive for virus detection than MSVC. MSVC was consistently one to two sera dilutions more sensitive than SCC for the detection of Flaviviruses from patients' sera. Both methods were approximately equally sensitive for the detection of Bunyaviruses from patients' sera and equal or one dilution less sensitive for the detection of Alphaviruses from patients' sera. Additionally, MSVC detected DEN virus in five of seven DEN-3 RT-PCR positive, SCC negative patients' sera. |
| Databáze: | OpenAIRE |
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