The cognitive impairment induced by zinc deficiency in rats aged 0∼2 months related to BDNF DNA methylation changes in the hippocampus

Autor: Yan-Qiang Liu, Wei Pang, Yugang Jiang, Yan-Dan Hu, Wei Liu, Cheng-Yu Huang, Hao Lu, Cong-cong He, Zi-Yu Wang
Rok vydání: 2016
Předmět:
DNA (Cytosine-5-)-Methyltransferase 1
Male
0301 basic medicine
medicine.medical_specialty
Offspring
Medicine (miscellaneous)
chemistry.chemical_element
Zinc
Biology
Hippocampus
DNA Methyltransferase 3A
Epigenesis
Genetic
Random Allocation
03 medical and health sciences
0302 clinical medicine
Lactation
Internal medicine
Avoidance Learning
medicine
Animals
Weaning
Hippocampus (mythology)
Cognitive Dysfunction
DNA (Cytosine-5-)-Methyltransferases
RNA
Messenger
Neurons
Brain-derived neurotrophic factor
Nutrition and Dietetics
Behavior
Animal
Brain-Derived Neurotrophic Factor
General Neuroscience
Gene Expression Regulation
Developmental
Exons
Maternal Nutritional Physiological Phenomena
General Medicine
DNA Methylation
medicine.disease
Rats
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
DNA methylation
Zinc deficiency
Female
Deficiency Diseases
030217 neurology & neurosurgery
Zdroj: Nutritional Neuroscience. 20:519-525
ISSN: 1476-8305
1028-415X
DOI: 10.1080/1028415x.2016.1194554
Popis: This study was carried out to understand the effects of zinc deficiency in rats aged 0∼2 months on learning and memory, and the brain-derived neurotrophic factor (BDNF) gene methylation status in the hippocampus.The lactating mother rats were randomly divided into three groups (n = 12): zinc-adequate group (ZA: zinc 30 mg/kg diet), zinc-deprived group (ZD: zinc 1 mg/kg diet), and a pair-fed group (PF: zinc 30 mg/kg diet), in which the rats were pair-fed to those in the ZD group. After weaning (on day 23), offspring were fed the same diets as their mothers. After 37 days, the zinc concentrations in the plasma and hippocampus were measured, and the behavioral function of the offspring rats was measured using the passive avoidance performance test. We then assessed the DNA methylation patterns of the exon IX of BDNF by methylation-specific quantitative real-time PCR and the mRNA expression of BDNF in the hippocampus by RT-PCR.Compared with the ZA and PF groups, rats in the ZD group had shorter latency period, lower zinc concentrations in the plasma and hippocampus (P 0.05). Interestingly, the DNA methylation of the BDNF exon IX was significantly increased in the ZD group, compared with the ZA and PF groups, whereas the expression of the BDNF mRNA was decreased. In addition, the DNMT1 mRNA expression was significantly upregulated and DNMT3A was downregulated in the ZD group, but not in the ZA and PF groups.The learning and memory damage in offspring may be a result of the epigenetic changes of the BDNF genes in response to the zinc-deficient diet during 0∼2 month period. Furthermore, this work supports the speculative notion that altered DNA methylation of BDNF in the hippocampus is one of the main causes of cognitive impairment by zinc deficiency.
Databáze: OpenAIRE
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