Extract of Emblica officinalis enhances the growth of human keratinocytes in culture
| Autor: | Dai Ayusawa, Yoshimi Yamakami, Ryuichiro Kasukabe, Mohammad Nazir Hossain, Yuki Takauji, Kensuke Miki, Kyoko Morino, Michihiko Fujii |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Keratinocytes
0211 other engineering and technologies Human skin Phyllanthus emblica 02 engineering and technology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine 021105 building & construction medicine Humans Growth Substances Chromatography High Pressure Liquid Cell Proliferation Epidermis (botany) Cell growth Plant Extracts Coomassie Brilliant Blue 030205 complementary & alternative medicine Staining medicine.anatomical_structure Complementary and alternative medicine chemistry Biochemistry Officinalis Keratinocyte Wound healing |
| Zdroj: | Journal of integrative medicine. 17(2) |
| ISSN: | 2095-4964 |
| Popis: | Objective Keratinocytes are the predominant cell type in the epidermis and play key roles in epidermal function. Thus, identification of the compounds that regulate the growth of keratinocytes is of importance. Here we searched for such compounds from the herbs used in traditional medicine Ayurveda. Methods Human keratinocytes were cultured in the presence or absence of the herbal extracts for 2 weeks; the effect of the extracts on cell growth was determined by staining the cells with Coomassie brilliant blue. To detect the compounds that regulate the growth of keratinocytes, the herbal extracts were subjected to high-performance liquid chromatography (HPLC). Results We found that the extract of Emblica officinalis enhanced the growth of keratinocytes in culture. Further, we fractionated the extract of E. officinalis using HPLC and identified the fractions responsible for the enhanced growth of keratinocytes. Conclusion The extract of E. officinalis enhanced the growth of human keratinocytes in culture. E. officinalis contains the compounds that would be beneficial for human skin health because enhanced growth of keratinocytes would promote wound healing. |
| Databáze: | OpenAIRE |
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