Mitochondrial DNA sequence variation in Drosophilid species (Diptera: Drosophilidae) along altitudinal gradient from Central Himalayan region of India
| Autor: | Saurabh Dewan, Manisha Sarswat, Rajendra S. Fartyal |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine Mitochondrial DNA Genetic Speciation Rare species India Zoology Biology DNA Mitochondrial 010603 evolutionary biology 01 natural sciences Electron Transport Complex IV 03 medical and health sciences Phylogenetics RNA Ribosomal 16S Genetic variation Botany Genetics Zaprionus Animals Drosophilidae Ecosystem Phylogeny Genetic diversity Base Sequence Altitude Cytochrome c oxidase subunit II Cytochrome c oxidase subunit I Genetic Variation biology.organism_classification Mitochondria Isoenzymes 030104 developmental biology Haplotypes Insect Proteins Female |
| Zdroj: | Journal of Genetics. 95:357-367 |
| ISSN: | 0973-7731 0022-1333 |
| Popis: | Central Himalayan region of India encompasses varied ecological habitats ranging from near tropics to the mid-elevation forests dominated by cool-temperate taxa. In past, we have reported several new records and novel species from Uttarakhand state of India. Here, we assessed genetic variations in three mitochondrial genes, namely, 16S rRNA, cytochrome c oxidase subunit I and cytochrome c oxidase subunit II (COI and COII) in 26 drosophilid species collected along altitudinal transect from 550 to 2700 m above mean sea level. In the present study, overall 543 sequences were generated, 82 for 16S rRNA, 238 for COI, 223 for COII with 21, 47 and 45 mitochondrial haplotypes for 16S rRNA, COI and COII genes, respectively. Almost all species were represented by 2-3 unique mitochondrial haplotypes, depicting a significant impact of environmental heterogeneity along altitudinal gradient on genetic diversity. Also for the first time, molecular data of some rare species like Drosophila mukteshwarensis, Liodrosophila nitida, Lordiphosa parantillaria, Lordiphosa ayarpathaensis, Scaptomyza himalayana, Scaptomyza tistai, Zaprionus grandis and Stegana minuta are provided to public domains through this study. |
| Databáze: | OpenAIRE |
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