QTL mapping uncovers a semi-dwarf 1 (sdw1) allele in the barley (Hordeum vulgare) ND23049 line
| Autor: | Stéphanie Paquet-Marceau, Juan E. Díaz Lago, François Belzile, Sébastien Bélanger |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genotyping by sequencing Genotype Quantitative Trait Loci Single-nucleotide polymorphism Haploidy Quantitative trait locus Biology Polymorphism Single Nucleotide 01 natural sciences 03 medical and health sciences Genetics Allele Molecular Biology Alleles Plant Proteins food and beverages Hordeum General Medicine Plant Breeding Phenotype 030104 developmental biology Agronomy Mutation Hordeum vulgare 010606 plant biology & botany Biotechnology |
| Zdroj: | Genome. 61:429-436 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/gen-2017-0211 |
| Popis: | In barley, semi-dwarf varieties are attractive for their superior harvest index and lodging resistance, but many semi-dwarf barley genotypes suffer from poor spike emergence. We performed a genetic characterization of a semi-dwarf line (ND23049) that combines short stature, strong stiff culms, and adequate spike emergence. We developed a doubled haploid (DH) population derived by crossing ND23049 and the cultivar CLE253. A subset of 88 DH lines and parents were characterized for plant height in 2013 and 2014 and genotyped. In total, 1984 SNPs (345 unique loci) were used to produce a linkage map of 1127.1 cM. Three QTLs for plant height were detected in this population and coincided with the HvGA20ox2/Sdw1, HvBRI1/Uzu1, and HvPRR95 gene loci. The phenotypic variation explained by each QTL was 75.8%, 7.7%, and 4.1%, respectively, and jointly explained 83.3% (2013) and 87.7% (2014) of plant height. Our results suggest that ND23049 contributed the “short” allele at the HvGA20ox2/sdw1 locus while CLE253 provided “short” alleles at the HvBRI1/uzu1 and HvPRR95 loci. We identified a large deletion (at least 92.7 Kb), including HvGA20ox2 (Sdw1), as the causal mutation in ND23049. A set of tightly flanked SNP markers will help breeders to develop improved semi-dwarf varieties. |
| Databáze: | OpenAIRE |
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