A novel microRNA IamiR-4-3p from water spinach (Ipomoea aquatica Forsk.) increased Cd uptake and translocation in Arabidopsis thaliana

Autor: Chuang Shen, Ying-Ying Huang, Jun-Liang Xin, Chun-Tao He, Zhong-Yi Yang
Rok vydání: 2021
Předmět:
Zdroj: Environmental science and pollution research international. 29(27)
ISSN: 1614-7499
Popis: MicroRNAs (miRNAs) play important roles in plant response to Cd stress. In our previous study, we observed significant differences in the expression levels of IamiR-4-3p between high-Cd and low-Cd cultivars of water spinach. The function of IamiR-4-3p was investigated by using wild type Arabidopsis (WT), Arabidopsis transfected with empty vector pCambia1302 (CK), and Arabidopsis transfected with IamiR-4-3p + vector pCambia1302 (p35S::miR-4-3p) in this study. In p35S::miR-4-3p Arabidopsis, the expression levels of GST3 and AWPM19-like were reduced by 20% and 24%. Under Cd treatment, higher root and shoot Cd concentrations were detected in the transgenic p35S::miR-4-3p Arabidopsis. MDA and H
Databáze: OpenAIRE