Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation
| Autor: | Rémi Dumollard, Isa D. L. Gomes, Celine Hebras, Alex McDougall |
|---|---|
| Přispěvatelé: | Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Cord Ascidian [SDV.BA]Life Sciences [q-bio]/Animal biology Phallusia mammillata Embryogenesis [SDV.BC]Life Sciences [q-bio]/Cellular Biology Gene editing Biology Microinjection Cell biology Nerve cord 03 medical and health sciences 0302 clinical medicine Genome editing CRISPR CRISPR-Cas9 030217 neurology & neurosurgery ComputingMilieux_MISCELLANEOUS 030304 developmental biology |
| Zdroj: | Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation., pp.217-230, 2021, ⟨10.1007/978-1-0716-0974-3_13⟩ Developmental Biology of the Sea Urchin and Other Marine Invertebrates Methods in Molecular Biology ISBN: 9781071609736 Methods in Molecular Biology Methods in Molecular Biology-Developmental Biology of the Sea Urchin and Other Marine Invertebrates Developmental Biology of the Sea Urchin and Other Marine Invertebrates, 2219, Springer US; Springer US, pp.217-230, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-0974-3_13,⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-0716-0974-3_13⟩ |
| Popis: | International audience; Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian Phallusia mammillata. |
| Databáze: | OpenAIRE |
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