Constitutive and inducible nitric oxide synthases after dynorphin-induced spinal cord injury
| Autor: | Na Liu, Wenhui Hu, Guo Qiang Wang, Wenan Qiang, Xuan-cai S.T. Wan, Jing Sheng Liu, Fang Li, Wei Hong Liao, Hai Yan Wang, Min Feng Jen |
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| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Transcription Genetic Arginine Nitric Oxide Synthase Type II Nitric Oxide Synthase Type I In situ hybridization Dynorphin Dynorphins Neuroprotection Gene Expression Regulation Enzymologic Nitric oxide Cellular and Molecular Neuroscience chemistry.chemical_compound Reference Values Internal medicine medicine Animals RNA Messenger Rats Wistar Spinal cord injury Spinal Cord Injuries biology Chemistry medicine.disease Spinal cord Immunohistochemistry Rats Nitric oxide synthase medicine.anatomical_structure Endocrinology Spinal Cord Anesthesia biology.protein Nitric Oxide Synthase |
| Zdroj: | Journal of Chemical Neuroanatomy. 17:183-197 |
| ISSN: | 0891-0618 |
| DOI: | 10.1016/s0891-0618(99)00039-3 |
| Popis: | It has recently been demonstrated that selective inhibition of both neuronal constitutive and inducible nitric oxide synthases (ncNOS and iNOS) is neuroprotective in a model of dynorphin (Dyn) A(1–17)-induced spinal cord injury. In the present study, various methods including the conversion of 3H- l -arginine to 3H-citrulline, immunohistochemistry and in situ hybridization are employed to determine the temporal profiles of the enzymatic activities, immunoreactivities, and mRNA expression for both ncNOS and iNOS after intrathecal injection of a neurotoxic dose (20 nmol) of Dyn A(1–17). The expression of ncNOS immunoreactivity and mRNA increased as early as 30 min after injection and persisted for 1–4 h. At 24–48 h, the number of ncNOS positive cells remained elevated while most neurons died. The cNOS enzymatic activity in the ventral spinal cord also significantly increased at 30 min–48 h, but no significant changes in the dorsal spinal cord were observed. However, iNOS mRNA expression increased later at 2 h, iNOS immunoreactivity and enzymatic activity increased later at 4 h and persisted for 24–48 h after injection of 20 nmol Dyn A(1–17). These results indicate that both ncNOS and iNOS are associated with Dyn-induced spinal cord injury, with ncNOS predominantly involved at an early stage and iNOS at a later stage. |
| Databáze: | OpenAIRE |
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