Distribution, evolution, and diversity of retrotransposons at the flamenco locus reflect the regulatory properties of piRNA clusters

Autor: Silke Jensen, Michael Coiffet, Isabelle Luyten, Matthias Zytnicki, Chantal Vaury, Hadi Quesneville, Vanessa Zanni, Angeline Eymery
Přispěvatelé: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique et Ecophysiologie de la qualité des agrumes (GEQA), Institut National de la Recherche Agronomique (INRA), Génétique, Reproduction et Développement (GReD ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Friedrich Miescher Institute for Biomedical Research, Unité de Recherche Génomique Info (URGI), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Génétique, Reproduction et Développement - Clermont Auvergne (GReD ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Vaury, Chantal, ProdInra, Archive Ouverte
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Transposable element
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Gene Transfer
Horizontal
Retroelements
Heterochromatin
Molecular Sequence Data
Oligonucleotides
Piwi-interacting RNA
Locus (genetics)
Retrotransposon
Biotechnologies
Evolution
Molecular
évolution structurale
03 medical and health sciences
gene silencing
0302 clinical medicine
Animals
Drosophila Proteins
RNA
Small Interfering
030304 developmental biology
Segmental duplication
Genetics
0303 health sciences
Multidisciplinary
Base Sequence
biology
epigenetics
fungi
food and beverages
Computational Biology
Genetic Variation
Sequence Analysis
DNA
RNAi
Biological Sciences
Cadherins
biology.organism_classification
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
Drosophila melanogaster
hétérochromatine
Horizontal gene transfer
RNA Interference
Sequence Alignment
rétrotransposon
030217 neurology & neurosurgery
Zdroj: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2013, ⟨10.1073/pnas.1313677110⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2013, ⟨10.1073/pnas.1313677110⟩
Proceedings of the National Academy of Sciences of the United States of America, 19842-19847. (2013)
ISSN: 0027-8424
1091-6490
DOI: 10.1073/pnas.1313677110⟩
Popis: article publié d'abord "online" PNAS early edition page 1à 6.; Most of our understanding of Drosophila heterochromatin structure and evolution has come from the annotation of heterochromatin from the isogenic y; cn bw sp strain. However, almost nothing is known about the heterochromatin's structural dynamics and evolution. Here, we focus on a 180-kb heterochromatic locus producing Piwi-interacting RNAs (piRNA cluster), the flamenco (flam) locus, known to be responsible for the control of at least three transposable elements (TEs). We report its detailed structure in three different Drosophila lines chosen according to their capacity to repress or not to repress the expression of two retrotransposons named ZAM and Idefix, and we show that they display high structural diversity. Numerous rearrangements due to homologous and nonhomologous recombination, deletions and segmental duplications, and loss and gain of TEs are diverse sources of active genomic variation at this locus. Notably, we evidence a correlation between the presence of ZAM and Idefix in this piRNA cluster and their silencing. They are absent from flam in the strain where they are derepressed. We show that, unexpectedly, more than half of the flam locus results from recent TE insertions and that most of the elements concerned are prone to horizontal transfer between species of the melanogaster subgroup. We build a model showing how such high and constant dynamics of a piRNA master locus open the way to continual emergence of new patterns of piRNA biogenesis leading to changes in the level of transposition control.
Databáze: OpenAIRE
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