Enzymatic determination of total 3 alpha-hydroxy bile acids in faeces. Validation in healthy subjects of a rapid method suitable for clinical routine purpose
| Autor: | N. F. Larusso, Einar Krag, S Arffmann, Axel Malchow-Møller |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Adult
medicine.medical_specialty 3-Hydroxysteroid Dehydrogenases Chromatography Gas medicine.drug_class Alpha (ethology) Biology Stool specimen Gastroenterology Bile Acids and Salts Feces Internal medicine medicine Humans Aged chemistry.chemical_classification Bile acid Healthy subjects Stool examination Clinical Enzyme Tests Middle Aged Clinical routine Enzyme chemistry |
| Zdroj: | Scandinavian journal of gastroenterology. 17(3) |
| ISSN: | 0036-5521 |
| Popis: | A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3 alpha-steroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (mean +/- S.D.) for faecal bile acid output was calculated to be 0-975 mumol/24h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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