Construction and characterization of monoclonal antibodies against western equine encephalitis virus
| Autor: | M C, Long, L P, Nagata, G V, Ludwig, A Z, Alvi, J D, Conley, A R, Bhatti, M R, Suresh, R E, Fulton |
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| Rok vydání: | 2000 |
| Předmět: |
Sindbis virus
animal structures medicine.drug_class viruses Immunology Blotting Western Enzyme-Linked Immunosorbent Assay Alphavirus Cross Reactions medicine.disease_cause Monoclonal antibody Virus Encephalitis Virus Western Equine Encephalitis Virus Venezuelan Equine Mice Antigen Genetics medicine Animals Antigens Viral Mice Inbred BALB C Western equine encephalitis virus Hybridomas biology virus diseases Antibodies Monoclonal biology.organism_classification Virology Precipitin Tests Immunoglobulin Isotypes Venezuelan equine encephalitis virus Togaviridae Encephalitis Virus Eastern Equine Sindbis Virus |
| Zdroj: | Hybridoma. 19(2) |
| ISSN: | 0272-457X |
| Popis: | A repertoire of mouse monoclonal antibodies (MAbs) against western equine encephalitis virus (WEE) was constructed and characterized. Anti-WEE antibodies were expressed from hybridomas and purified by protein G chromatography. Each of the antibodies was functionally assessed by indirect enzyme-linked immunosorbent assays (ELISAs), Western blotting, and immunoprecipitations. All antibodies bound to WEE antigen in ELISAs, whereas only a subgroup of antibodies was found to be active in Western blotting and immunoprecipitations. A subset of antibodies was found to cross-react with other alphaviruses, such as Sindbis virus (SIN), Venezuelan equine encephalitis (VEE), and eastern equine encephalitis (EEE). Because many of the antibodies were highly reactive to WEE antigen in one or more of the assays, these antibodies are excellent candidates for immunodetection and immunotherapy studies. |
| Databáze: | OpenAIRE |
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