Low genetic diversity and complexity of submicroscopic Plasmodium falciparum infections among febrile patients in low transmission areas in Senegal

Autor: Babacar Diouf, Cheikh Talla, Nafissatou Diagne, Abdoulaye Badiane, Cheikh Sokhna, Fatoumata Diene Sarr, Aissatou Toure-Balde, Rokhaya Sane, Makhtar Niang, Pape Mbacké Sembène, Joseph Faye
Přispěvatelé: Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Institut Pasteur de Dakar and Institute for Research and Development of Senegal, Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Veterinary medicine
Plasmodium
Population genetics
Protozoan Proteins
0302 clinical medicine
Human genetics
Polymorphism (computer science)
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Malaria
Falciparum
Child
Merozoite Surface Protein 1
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Multidisciplinary
biology
Parasitic diseases
Senegal
3. Good health
Exact test
Carrier State
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Medicine
Female
Research Article
Adolescent
Fever
Science
030231 tropical medicine
Plasmodium falciparum
Antigens
Protozoan
Young Adult
03 medical and health sciences
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Malarial parasites
parasitic diseases
medicine
Humans
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Allele
Genotyping
Genetic diversity
Polymorphism
Genetic
biology.organism_classification
medicine.disease
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Malaria
030104 developmental biology
Carriage
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2019, 14 (4), pp.e0215755. ⟨10.1371/journal.pone.0215755⟩
PLoS ONE, 2019, 14 (4), pp.e0215755. ⟨10.1371/journal.pone.0215755⟩
PLoS ONE, Vol 14, Iss 4, p e0215755 (2019)
ISSN: 1932-6203
Popis: IntroductionSubmicroscopic Plasmodium infections are common in malaria endemic countries, but very little studies have been done in Senegal. This study investigates the genetic diversity and complexity of submicroscopic P. falciparum infections among febrile patients in low transmission areas in Senegal.Materials and methodsHundred and fifty blood samples were collected from febrile individuals living in Dielmo and Ndiop (Senegal) between August 2014 and January 2015, tested for microscopic and sub-microscopic P. falciparum infections and characterized for their genetic diversity and complexity of infections using msp-1 and msp-2 genotyping.ResultsSubmicroscopic P. falciparum infections were 19.6% and 25% in Dielmo and Ndiop, respectively. K1 and 3D7 were the predominant msp-1 and msp-2 allelic types with respective frequencies of 67.36% and 67.10% in microscopic isolates and 58.24% and 78% in submicroscopic ones. Frequencies of msp-1 allelic types were statistically comparable between the studied groups (p>0.05), and were respectively 93.54% vs 87.5% for K1, 60% vs 54.83% for MAD20 and 41.93% vs 22.5% for RO33 while frequencies of msp-2 allelic types were significantly highest in the microscopy group for FC27 (41.93% vs 10%, Fisher's Exact Test, p = 0.001) and 3D7 (61.29% vs 32.5%, Fisher's Exact Test, p = 0.02). Multiplicities of infection were lowest in submicroscopic P. falciparum isolates.ConclusionsThe study revealed a high submicroscopic P. falciparum carriage among patients in the study areas, and that submicroscopic P. falciparum isolates had a lower genetic diversity and complexity of malaria infections.
Databáze: OpenAIRE
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