Expression Profiling of Nonpolar Lipids in Meibum From Patients With Dry Eye: A Pilot Study
| Autor: | Kelly K. Nichols, Jeremy Keirsey, Kari B. Green, Jianzhong Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Spectrometry Mass Electrospray Ionization Electrospray ionization Dry Eye Syndromes Meibomian gland Very long chain Pilot Projects meibum Biology Bioinformatics Cornea 03 medical and health sciences 0302 clinical medicine Internal medicine triacylglycerols medicine Humans Statistical analysis dry eyes mass spectrometry Postmenopausal women Meibomian Glands Middle Aged Lipids 3. Good health Gene expression profiling Contact lens 030104 developmental biology Endocrinology medicine.anatomical_structure Tears 030221 ophthalmology & optometry Female |
| Zdroj: | Investigative Ophthalmology & Visual Science |
| ISSN: | 1552-5783 0146-0404 |
| Popis: | Purpose The purpose of this investigation was to characterize differentially expressed lipids in meibum samples from patients with dry eye disease (DED) in order to better understand the underlying pathologic mechanisms. Methods Meibum samples were collected from postmenopausal women with DED (PW-DED; n = 5) and a control group of postmenopausal women without DED (n = 4). Lipid profiles were analyzed by direct infusion full-scan electrospray ionization mass spectrometry (ESI-MS). An initial analysis of 145 representative peaks from four classes of lipids in PW-DED samples revealed that additional manual corrections for peak overlap and isotopes only slightly affected the statistical analysis. Therefore, analysis of uncorrected data, which can be applied to a greater number of peaks, was used to compare more than 500 lipid peaks common to PW-DED and control samples. Statistical analysis of peak intensities identified several lipid species that differed significantly between the two groups. Data from contact lens wearers with DED (CL-DED; n = 5) were also analyzed. Results Many species of the two types of diesters (DE) and very long chain wax esters (WE) were decreased by ∼20% in PW-DED, whereas levels of triacylglycerols were increased by an average of 39% ± 3% in meibum from PW-DED compared to that in the control group. Approximately the same reduction (20%) of similar DE and WE was observed for CL-DED. Conclusions Statistical analysis of peak intensities from direct infusion ESI-MS results identified differentially expressed lipids in meibum from dry eye patients. Further studies are warranted to support these findings. |
| Databáze: | OpenAIRE |
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