Allorhizobium undicola gen. nov., sp. nov., nitrogen-fixing bacteria that efficiently nodulate Neptunia natans in Senegal
| Autor: | Anne Willems, Matthew D. Collins, Renata Coopman, Monique Gillis, Bernard Dreyfus, P. de Lajudie, E Laurent-Fulele, U Torck, Karel Kersters |
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| Předmět: |
Agrobacterium vitis
ESPECE NOUVELLE Molecular Sequence Data Immunology BACTERIE TAXONOMIE Polymerase Chain Reaction Microbiology Bradyrhizobium GENETIQUE DE POPULATION Rhizobiaceae 23S ribosomal RNA Nitrogen Fixation RNA Ribosomal 16S Terminology as Topic Botany PHYLOGENIE SYMBIOSE Internal transcribed spacer Symbiosis Phylogeny LEGUMINEUSE TROPICALE Base Composition Plants Medicinal biology FIXATION BIOLOGIQUE DE L'AZOTE Mesorhizobium Nucleic Acid Hybridization food and beverages Fabaceae Genes rRNA Sequence Analysis DNA biology.organism_classification Senegal RNA Ribosomal 23S NODULE CAULINAIRE Phenotype Sinorhizobium Azorhizobium Carbohydrate Metabolism Rhizobium Electrophoresis Polyacrylamide Gel GENRE NOUVEAU Polymorphism Restriction Fragment Length |
| Zdroj: | ResearcherID |
| Popis: | A group of nodule isolates form #Neptunia natans$, an indigenous stem-nodulated tropical legume found in waterlogged areas of Senegal, was studied. Polyphasic taxonomy was performed, including SDS-PAGE of total proteins, auxanography using API galleries, host-plant specificity, PCR-RFLP of the internal transcribed spacer region between the 16S and the 23S rRNA coding genes, 16S rRNA gene sequencing and DNA-DNA hybridization. It was demonstrated that this group is phenotypically and phylogenetically separate from the known species of #Rhizobium$, Sinorhizobium$, #Mesorhizobium$, #Agrobacterium$, #Bradyrhizobium$, and #Azorhizobium$. Its closest phylogenetic neighbour, as deduced by 16S rRNA gene sequencing, is #Agrobacterium vitis$ (96.2% sequence homology). The name #Allorhizobium undicola$ gen. nov., sp. nov., is proposed for this group of bacteria, which are capable of efficient nitrogen-fixing symbiosis with #Neptunia natans$, and the type strain is ORS 9925T) (= LMG 11875(T)). (Résumé d'auteur) |
| Databáze: | OpenAIRE |
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