Enantioselective separation of biologically active basic compounds in ultra-performance supercritical fluid chromatography
| Autor: | Květa Kalíková, Radim Geryk, Eva Tesařová, Martin G. Schmid |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Chromatography
010405 organic chemistry 010401 analytical chemistry Temperature Enantioselective synthesis Chromatography Supercritical Fluid Stereoisomerism Biological activity Chiral stationary phase 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Amphetamine chemistry.chemical_compound Alkaloids chemistry Phase (matter) Pressure Supercritical fluid chromatography Trifluoroacetic acid Environmental Chemistry Isopropylamine Spectroscopy Derivative (chemistry) |
| Zdroj: | Analytica Chimica Acta. 932:98-105 |
| ISSN: | 0003-2670 |
| Popis: | The enantioseparation of basic compounds represent a challenging task in modern SFC. Therefore this work is focused on development and optimization of fast SFC methods suitable for enantioseparation of 27 biologically active basic compounds of various structures. The influences of the co-solvent type as well as different mobile phase additives on retention, enantioselectivity and enantioresolution were investigated. Obtained results confirmed that the mobile phase additives, especially bases (or the mixture of base and acid), improve peak shape and enhance enantioresolution. The best results were achieved with isopropylamine or the mixture of isopropylamine and trifluoroacetic acid as additives. In addition, the effect of temperature and back pressure were evaluated to optimize the enantioseparation process. The immobilized amylose-based chiral stationary phase, i.e. tris(3,5-dimethylphenylcarbamate) derivative of amylose proved to be useful tool for the enantioseparation of a broad spectrum of chiral bases. The chromatographic conditions that yielded baseline enantioseparations of all tested compounds were discovered. The presented work can serve as a guide for simplifying the method development for enantioseparation of basic racemates in SFC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect