Decreased haem oxygenase-1 and increased inducible nitric oxide synthase in the lung of severe COPD patients
| Autor: | Piero Maestrelli, Yohann Nowicki, Graziella Turato, Bruno Formichi, C. Paska, Massimo Miniati, Leonardo M. Fabbri, Marina Saetta, Simonetta Monti |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine medicine.medical_specialty Nitric Oxide Synthase Type II Inflammation medicine.disease_cause Severity of Illness Index Nitric oxide Pulmonary Disease Chronic Obstructive chemistry.chemical_compound Internal medicine Macrophages Alveolar medicine Humans Lung Aged COPD biology business.industry Nitrotyrosine Respiratory disease Membrane Proteins Middle Aged medicine.disease Respiratory Function Tests Nitric oxide synthase Oxidative Stress cigarette smoke immunohistochemistry inflammation macrophages oxidative stress Endocrinology medicine.anatomical_structure chemistry Heme Oxygenase (Decyclizing) Immunology biology.protein Tyrosine Female Nitric Oxide Synthase medicine.symptom business Heme Oxygenase-1 Oxidative stress |
| Zdroj: | Scopus-Elsevier |
| Popis: | Oxidant/antioxidant imbalance is implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The current study examined the expression of antioxidant and pro-oxidant enzymes, haem oxygenases (HO) and inducible nitric oxide synthase (iNOS) respectively, in patients with severe COPD and control smokers without lung function impairment. Immunoreactivity for HO-1, HO-2, iNOS and nitric oxide-derived oxidants expressed as nitrotyrosine (N-Tyr) was quantified in peripheral lung. HO-1+ alveolar macrophages were decreased in severe COPD compared to control smokers, whereas no difference was observed in iNOS+ macrophages. In contrast, severe patients had significantly higher numbers of iNOS+ cells in alveolar walls. These iNOS+ cells were identified as type 2 pneumocytes and their number was inversely related to HO-1+ macrophages. There were no significant differences in N-Tyr immunostaining between the two groups. However, the rate of protein nitration in lung tissue was directly related to iNOS expression and associated with lower values of forced expiratory volume in one second/forced vital capacity. HO-2 was constitutively expressed by type 2 pneumocytes and these cells were increased in severe COPD. In conclusion, the results suggest that the enzymes involved in the oxidative stress response may have a different role in the lung defence and that imbalance between haem oxygenase-1 and inducible nitric oxide synthase may be associated with the development of severe impairment in chronic obstructive pulmonary disease patients. |
| Databáze: | OpenAIRE |
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