Chemoresistance acquisition induces a global shift of expression of aniogenesis-associated genes and increased pro-angogenic activity in neuroblastoma cells

Autor: Michaelis, Martin, Klassert, Denise, Barth, Susanne, Suhan, Tatyana, Breitling, Rainer, Mayer, Bernd, Hinsch, Nora, Doerr, Hans W., Cinatl, Jaroslav, Cinatl, Jindrich, Cinatl jr., Jindrich
Přispěvatelé: Bioinformatics
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Cancer Research
Chick Embryo
Chorioallantoic Membrane
BLOOD-VESSELS
Mice
Neuroblastoma
VEGF EXPRESSION
Cluster Analysis
CYTOKINE NETWORK
Extracellular Signal-Regulated MAP Kinases
IN-VIVO
Oligonucleotide Array Sequence Analysis
Antibiotics
Antineoplastic
Neovascularization
Pathologic
TUMOR-GROWTH
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Phenotype
Gene Expression Regulation
Neoplastic
Oncology
Molecular Medicine
Female
Mice
Nude
Mice
Inbred Strains
Biology
lcsh:RC254-282
Cell Line
Cell Line
Tumor
EXTRACELLULAR-MATRIX
medicine
Animals
Humans
ddc:610
Cell Proliferation
MELANOMA-CELLS
N-MYC
Cell growth
Gene Expression Profiling
Research
Computational Biology
Endothelial Cells
ANGIOGENIC FACTORS
medicine.disease
Xenograft Model Antitumor Assays
In vitro
Gene expression profiling
Cell culture
Doxorubicin
Drug Resistance
Neoplasm
Culture Media
Conditioned
ENDOTHELIAL GROWTH-FACTOR
Cancer cell
Cancer research
N-Myc
Zdroj: Molecular Cancer, Vol 8, Iss 1, p 80 (2009)
Molecular Cancer
Molecular Cancer, 8(1):80. BMC
ISSN: 1476-4598
Popis: Background Chemoresistance acquisition may influence cancer cell biology. Here, bioinformatics analysis of gene expression data was used to identify chemoresistance-associated changes in neuroblastoma biology. Results Bioinformatics analysis of gene expression data revealed that expression of angiogenesis-associated genes significantly differs between chemosensitive and chemoresistant neuroblastoma cells. A subsequent systematic analysis of a panel of 14 chemosensitive and chemoresistant neuroblastoma cell lines in vitro and in animal experiments indicated a consistent shift to a more pro-angiogenic phenotype in chemoresistant neuroblastoma cells. The molecular mechanims underlying increased pro-angiogenic activity of neuroblastoma cells are individual and differ between the investigated chemoresistant cell lines. Treatment of animals carrying doxorubicin-resistant neuroblastoma xenografts with doxorubicin, a cytotoxic drug known to exert anti-angiogenic activity, resulted in decreased tumour vessel formation and growth indicating chemoresistance-associated enhanced pro-angiogenic activity to be relevant for tumour progression and to represent a potential therapeutic target. Conclusion A bioinformatics approach allowed to identify a relevant chemoresistance-associated shift in neuroblastoma cell biology. The chemoresistance-associated enhanced pro-angiogenic activity observed in neuroblastoma cells is relevant for tumour progression and represents a potential therapeutic target.
Databáze: OpenAIRE
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